2ru5: Difference between revisions
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''' | ==Designed Armadillo Repeat Protein Fragment (MAII)== | ||
<StructureSection load='2ru5' size='340' side='right' caption='[[2ru5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ru5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RU5 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ru4|2ru4]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ru5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ru5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ru5 RCSB], [http://www.ebi.ac.uk/pdbsum/2ru5 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Repeat proteins are built of modules, each of which constitutes a structural motif. We have investigated whether fragments of a designed consensus armadillo repeat protein (ArmRP) recognize each other. We examined a split ArmRP consisting of an N-capping repeat (denoted Y), three internal repeats (M), and a C-capping repeat (A). We demonstrate that the C-terminal MA fragment adopts a fold similar to the corresponding part of the entire protein. In contrast, the N-terminal YM2 fragment constitutes a molten globule. The two fragments form a 1:1 YM2:MA complex with a nanomolar dissociation constant essentially identical to the crystal structure of the continuous YM3A protein. Molecular dynamics simulations show that the complex is structurally stable over a 1 mus timescale and reveal the importance of hydrophobic contacts across the interface. We propose that the existence of a stable complex recapitulates possible intermediates in the early evolution of these repeat proteins. | |||
Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure.,Watson RP, Christen MT, Ewald C, Bumbak F, Reichen C, Mihajlovic M, Schmidt E, Guntert P, Caflisch A, Pluckthun A, Zerbe O Structure. 2014 Jul 8;22(7):985-95. doi: 10.1016/j.str.2014.05.002. Epub 2014 Jun, 12. PMID:24931467<ref>PMID:24931467</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Christen, M T.]] | |||
[[Category: Plueckthun, A.]] | |||
[[Category: Watson, R P.]] | |||
[[Category: Zerbe, O.]] | |||
[[Category: Armadillo repeat motif]] | |||
[[Category: De novo protein]] | |||
[[Category: Solenoid repeat]] | |||
Revision as of 07:51, 23 July 2014
Designed Armadillo Repeat Protein Fragment (MAII)
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