2bka: Difference between revisions

CC3 (TIP30) is a protein with pro-apoptotic and anti-metastatic, properties. The tumor suppressor effect of CC3 has been suggested to, result from inhibition of nuclear transport by binding to importin betas, or by regulating transcription through interaction in a complex with, co-activator independent of AF-2 function (CIA) and the c-myc gene., Previous biochemical studies indicated that CC3 has protein kinase, activity, and a structural similarity to cAMP-dependent protein kinase, catalytic subunit was proposed. By contrast, bioinformatics studies, suggested a relationship of CC3 to the short chain dehydrogenase reductase, family. To clarify details of the CC3 structural family and ligand binding, properties, we have determined the crystal structure of CC3 at 1.7-A, resolution. CC3 has a short chain dehydrogenase reductase fold and binding, specificity for NADPH, yet it is unlikely to be normally enzymatically, active because it is monomeric. These structural results, in conjunction, with data from earlier mutagenesis work on the nucleotide binding motif, suggest that NADPH binding is important for the biological activity of, CC3, including interaction with importins and with the CIA/c-myc system., CC3 provides an example of the adaptation of a metabolic enzyme fold to, include a regulatory role, as also seen in the case of the NADH-binding, co-repressor CtBP.

2BKA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4, NDP, PE8 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BKA OCA].  

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:13:05 2007''