4njg: Difference between revisions
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==Crystal Structure of QueE from Burkholderia multivorans in complex with AdoMet and 6-carboxypterin== | |||
<StructureSection load='4njg' size='340' side='right' caption='[[4njg]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4njg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burm1 Burm1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJG FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HHS:6-CARBOXYPTERIN'>HHS</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4njh|4njh]], [[4nji|4nji]], [[4njj|4njj]], [[4njk|4njk]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">queE, Bmul_3115, BMULJ_00116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395019 BURM1])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/7-carboxy-7-deazaguanine_synthase 7-carboxy-7-deazaguanine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.99.3 4.3.99.3] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4njg RCSB], [http://www.ebi.ac.uk/pdbsum/4njg PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/A9AC61_BURM1 A9AC61_BURM1]] Catalyzes the conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) (By similarity).[HAMAP-Rule:MF_00917] | [[http://www.uniprot.org/uniprot/A9AC61_BURM1 A9AC61_BURM1]] Catalyzes the conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) (By similarity).[HAMAP-Rule:MF_00917] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
7-carboxy-7-deazaguanine synthase (QueE) catalyzes a key S-adenosyl-L-methionine (AdoMet)- and Mg(2+)-dependent radical-mediated ring contraction step, which is common to the biosynthetic pathways of all deazapurine-containing compounds. QueE is a member of the AdoMet radical superfamily, which employs the 5'-deoxyadenosyl radical from reductive cleavage of AdoMet to initiate chemistry. To provide a mechanistic rationale for this elaborate transformation, we present the crystal structure of a QueE along with structures of pre- and post-turnover states. We find that substrate binds perpendicular to the [4Fe-4S]-bound AdoMet, exposing its C6 hydrogen atom for abstraction and generating the binding site for Mg(2+), which coordinates directly to the substrate. The Burkholderia multivorans structure reported here varies from all other previously characterized members of the AdoMet radical superfamily in that it contains a hypermodified (beta6/alpha3) protein core and an expanded cluster-binding motif, CX14CX2C. | |||
Radical SAM enzyme QueE defines a new minimal core fold and metal-dependent mechanism.,Dowling DP, Bruender NA, Young AP, McCarty RM, Bandarian V, Drennan CL Nat Chem Biol. 2014 Feb;10(2):106-12. doi: 10.1038/nchembio.1426. Epub 2013 Dec, 22. PMID:24362703<ref>PMID:24362703</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: 7-carboxy-7-deazaguanine synthase]] | [[Category: 7-carboxy-7-deazaguanine synthase]] | ||
[[Category: Bandarian, V | [[Category: Burm1]] | ||
[[Category: Bruender, N A | [[Category: Bandarian, V]] | ||
[[Category: Dowling, D P | [[Category: Bruender, N A]] | ||
[[Category: Drennan, C L | [[Category: Dowling, D P]] | ||
[[Category: McCarty, R M | [[Category: Drennan, C L]] | ||
[[Category: Young, A P | [[Category: McCarty, R M]] | ||
[[Category: Young, A P]] | |||
[[Category: Adomet radical enzyme]] | [[Category: Adomet radical enzyme]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
Revision as of 19:19, 25 December 2014
Crystal Structure of QueE from Burkholderia multivorans in complex with AdoMet and 6-carboxypterin
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