2bky: Difference between revisions
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==Overview== | ==Overview== | ||
Organisms growing at elevated temperatures face a particular challenge to, maintain the integrity of their genetic material. All thermophilic and, hyperthermophilic archaea encode one or more copies of the Alba (Sac10b), gene. Alba is an abundant, dimeric, highly basic protein that binds, cooperatively and at high density to DNA. Sulfolobus solfataricus encodes, a second copy of the Alba gene, and the Alba2 protein is expressed at, approximately 5% of the level of Alba1. We demonstrate by NMR, ITC, and, crystallography that Alba2 exists exclusively as a heterodimer with Alba1, at physiological concentrations and that heterodimerization exerts a clear, effect upon the DNA packaging, as observed by EM, potentially by changing, the interface between adjacent Alba dimers in DNA complexes. A . | Organisms growing at elevated temperatures face a particular challenge to, maintain the integrity of their genetic material. All thermophilic and, hyperthermophilic archaea encode one or more copies of the Alba (Sac10b), gene. Alba is an abundant, dimeric, highly basic protein that binds, cooperatively and at high density to DNA. Sulfolobus solfataricus encodes, a second copy of the Alba gene, and the Alba2 protein is expressed at, approximately 5% of the level of Alba1. We demonstrate by NMR, ITC, and, crystallography that Alba2 exists exclusively as a heterodimer with Alba1, at physiological concentrations and that heterodimerization exerts a clear, effect upon the DNA packaging, as observed by EM, potentially by changing, the interface between adjacent Alba dimers in DNA complexes. A functional, role for Alba2 in modulation of higher order chromatin structure and DNA, condensation is suggested. | ||
==About this Structure== | ==About this Structure== | ||
2BKY is a | 2BKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BKY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rna-binding]] | [[Category: rna-binding]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:13:14 2007'' | ||
