3zgh: Difference between revisions

Line 1:

~~{{STRUCTURE_3zgh| PDB=3zgh | SCENE= }}~~

==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP==

===Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP===

~~{{ABSTRACT_PUBMED_24430336}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[3zgh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGH FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zgi|3zgi]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zgh RCSB], [http://www.ebi.ac.uk/pdbsum/3zgh PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 A resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended beta-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short beta-strands, for interaction with KRT10.

~~==About this Structure==~~

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.,Schulte T, Lofling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A Open Biol. 2014 Jan 15;4(1):130090. doi: 10.1098/rsob.130090. PMID:24430336<ref>PMID:24430336</ref>

~~[[3zgh]] is~~ a 1 ~~chain structure~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3ZGH OCA].~~

==~~Reference~~==

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~<ref group="xtra">PMID:024430336</ref>~~<references ~~group="xtra"~~/><~~references~~/>

</div>

[[Category: Achour, A.]]

== References ==

[[Category: Diamante, A.]]

[[Category: Ebel, C.]]

__TOC__

[[Category: Henriques-Normark, B.]]

</StructureSection>

[[Category: Hentrich, K.]]

[[Category: Strpn]]

[[Category: Kikhney, A.]]

[[Category: Achour, A]]

[[Category: Loefling, J.]]

[[Category: Diamante, A]]

[[Category: Mikaelsson, C.]]

[[Category: Ebel, C]]

[[Category: Normark, S.]]

[[Category: Henriques-Normark, B]]

[[Category: Schulte, T.]]

[[Category: Hentrich, K]]

[[Category: Svergun, D.]]

[[Category: Kikhney, A]]

[[Category: Loefling, J]]

[[Category: Mikaelsson, C]]

[[Category: Normark, S]]

[[Category: Schulte, T]]

[[Category: Svergun, D]]

[[Category: Keratin-10]]

[[Category: Mscramm]]

[[Category: Structural protein]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_3zgh|  PDB=3zgh  |  SCENE=  }}
+==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP==
-===Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP===
+<StructureSection load='3zgh' size='340' side='right' caption='[[3zgh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24430336}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3zgh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGH FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zgi|3zgi]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zgh RCSB], [http://www.ebi.ac.uk/pdbsum/3zgh PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 A resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended beta-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short beta-strands, for interaction with KRT10.
-==About this Structure==
+The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.,Schulte T, Lofling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A Open Biol. 2014 Jan 15;4(1):130090. doi: 10.1098/rsob.130090. PMID:24430336<ref>PMID:24430336</ref>
-[[3zgh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGH OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024430336</ref><references group="xtra"/><references/>
+</div>
-[[Category: Achour, A.]]
+== References ==
-[[Category: Diamante, A.]]
+<references/>
-[[Category: Ebel, C.]]
+__TOC__
-[[Category: Henriques-Normark, B.]]
+</StructureSection>
-[[Category: Hentrich, K.]]
+[[Category: Strpn]]
-[[Category: Kikhney, A.]]
+[[Category: Achour, A]]
-[[Category: Loefling, J.]]
+[[Category: Diamante, A]]
-[[Category: Mikaelsson, C.]]
+[[Category: Ebel, C]]
-[[Category: Normark, S.]]
+[[Category: Henriques-Normark, B]]
-[[Category: Schulte, T.]]
+[[Category: Hentrich, K]]
-[[Category: Svergun, D.]]
+[[Category: Kikhney, A]]
+[[Category: Loefling, J]]
+[[Category: Mikaelsson, C]]
+[[Category: Normark, S]]
+[[Category: Schulte, T]]
+[[Category: Svergun, D]]
 [[Category: Keratin-10]]
 [[Category: Mscramm]]
 [[Category: Structural protein]]