3b31: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:3b31.jpg|left|200px]] | [[Image:3b31.jpg|left|200px]] | ||
'''Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA''' | {{Structure | ||
|PDB= 3b31 |SIZE=350|CAPTION= <scene name='initialview01'>3b31</scene>, resolution 2.40Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=IRI:IRIDIUM HEXAMMINE ION'>IRI</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
3B31 is a [ | 3B31 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B31 OCA]. | ||
==Reference== | ==Reference== | ||
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http:// | tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157151 18157151] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Costantino, D A.]] | [[Category: Costantino, D A.]] | ||
| Line 19: | Line 28: | ||
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: hybrid state]] | [[Category: hybrid state]] | ||
[[Category: | [[Category: ire]] | ||
[[Category: rna structure]] | [[Category: rna structure]] | ||
[[Category: translation initiation]] | [[Category: translation initiation]] | ||
| Line 25: | Line 34: | ||
[[Category: trna mimicry]] | [[Category: trna mimicry]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:44 2008'' | ||
Revision as of 16:55, 20 March 2008
| |||||||||||||
| 3b31, resolution 2.40Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | PO4 and IRI | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
Overview
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines.
About this Structure
3B31 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:18157151
Page seeded by OCA on Thu Mar 20 18:55:44 2008
