4n5q: Difference between revisions

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{{STRUCTURE_4n5q|  PDB=4n5q  |  SCENE=  }}
==Crystal structure of the N-terminal ankyrin repeat domain of TRPV3==
===Crystal structure of the N-terminal ankyrin repeat domain of TRPV3===
<StructureSection load='4n5q' size='340' side='right' caption='[[4n5q]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
{{ABSTRACT_PUBMED_24248473}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4n5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N5Q FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trpv3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n5q RCSB], [http://www.ebi.ac.uk/pdbsum/4n5q PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In all six members of TRPV channel subfamily, there is an ankyrin repeat domain (ARD) in their intracellular N-termini. Ankyrin (ANK) repeat, a common motif with typically 33 residues in each repeat, is primarily involved in protein-protein interactions. Despite the sequence similarity among the ARDs of TRPV channels, the structure of TRPV3-ARD, however, remains unknown. Here, we report the crystal structure of TRPV3-ARD solved at 1.95 A resolution, which reveals six-ankyrin repeats. While overall structure of TRPV3-ARD is similar to ARDs from other members of TRPV subfamily; it, however, features a noticeable finger 3 loop that bends over and is stabilized by a network of hydrogen bonds and hydrophobic packing, instead of being flexible as seen in known TRPV-ARD structures. Electrophysiological recordings demonstrated that mutating key residues R225, R226, Q255, and F249 of finger 3 loop altered the channel activities and pharmacology. Taken all together, our findings show that TRPV3-ARD with characteristic finger 3 loop likely plays an important role in channel function and pharmacology.


==Function==
Crystal structure of the N-terminal ankyrin repeat domain of TRPV3 reveals unique conformation of finger 3 loop critical for channel function.,Shi DJ, Ye S, Cao X, Zhang R, Wang K Protein Cell. 2013 Dec;4(12):942-50. doi: 10.1007/s13238-013-3091-0. Epub 2013, Nov 18. PMID:24248473<ref>PMID:24248473</ref>
[[http://www.uniprot.org/uniprot/TRPV3_MOUSE TRPV3_MOUSE]] Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity).


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4n5q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N5Q OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024248473</ref><references group="xtra"/><references/>
__TOC__
[[Category: Cao, X.]]
</StructureSection>
[[Category: Shi, D J.]]
[[Category: Lk3 transgenic mice]]
[[Category: Wang, K W.]]
[[Category: Cao, X]]
[[Category: Ye, S.]]
[[Category: Shi, D J]]
[[Category: Zhang, R.]]
[[Category: Wang, K W]]
[[Category: Ye, S]]
[[Category: Zhang, R]]
[[Category: Ankyrin]]
[[Category: Ankyrin]]
[[Category: Channel]]
[[Category: Channel]]
[[Category: Protein binding]]
[[Category: Protein binding]]
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