2bn0: Difference between revisions

Banana lectin (Banlec) is a dimeric plant lectin from the jacalin-related, lectin family. Banlec belongs to a subgroup of this family that binds to, glucose/mannose, but is unique in recognizing internal alpha1,3 linkages, as well as beta1,3 linkages at the reducing termini. Here we present the, crystal structures of Banlec alone and with laminaribiose (LAM) (Glcbeta1, 3Glc) and Xyl-beta1,3-Man-alpha-O-Methyl. The structure of Banlec has a, beta-prism-I fold, similar to other family members, but differs from them, in its mode of sugar binding. The reducing unit of the sugar is inserted, into the binding site causing the second saccharide unit to be placed in, the opposite orientation compared with the other ligand-bound structures, of family members. More importantly, our structures reveal the presence of, a second sugar binding site that has not been previously reported in the, literature. The residues involved in the second site are common to other, lectins in this family, potentially signaling a new group of, mannose-specific jacalin-related lectins (mJRL) with two sugar binding, sites.

2BN0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musa_acuminata Musa acuminata] with CD and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BN0 OCA].  

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