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==D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoheptaose== | |||
<StructureSection load='3wnm' size='340' side='right' caption='[[3wnm]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wnm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WNM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wnk|3wnk]], [[3wnl|3wnl]], [[3wnn|3wnn]], [[3wno|3wno]], [[3wnp|3wnp]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnm RCSB], [http://www.ebi.ac.uk/pdbsum/3wnm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His(6) tag diffracted to a resolution of 2.3 A and belonged to space group P3(1)21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 A and belonged to space group P2(1)2(1)2(1), containing two molecules in the asymmetric unit. | |||
Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050<ref>PMID:23908050</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Atcc 24]] | |||
[[Category: Cycloisomaltooligosaccharide glucanotransferase]] | [[Category: Cycloisomaltooligosaccharide glucanotransferase]] | ||
[[Category: Fujimoto, Z | [[Category: Fujimoto, Z]] | ||
[[Category: Funane, K | [[Category: Funane, K]] | ||
[[Category: Kim, Y M | [[Category: Kim, Y M]] | ||
[[Category: Kimura, A | [[Category: Kimura, A]] | ||
[[Category: Kishine, N | [[Category: Kishine, N]] | ||
[[Category: Kitamura, S | [[Category: Kitamura, S]] | ||
[[Category: Kobayashi, M | [[Category: Kobayashi, M]] | ||
[[Category: Momma, M | [[Category: Momma, M]] | ||
[[Category: Suzuki, N | [[Category: Suzuki, N]] | ||
[[Category: Suzuki, R | [[Category: Suzuki, R]] | ||
[[Category: Suzuki, S | [[Category: Suzuki, S]] | ||
[[Category: 6-glucan]] | [[Category: 6-glucan]] | ||
[[Category: Alpha-1]] | [[Category: Alpha-1]] | ||
Revision as of 16:38, 21 December 2014
D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoheptaose
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