3boy: Difference between revisions
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'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA''' | {{Structure | ||
|PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene> | |||
|ACTIVITY= | |||
|GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |||
}} | |||
'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
3BOY is a [ | 3BOY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry 2GZT. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http:// | Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242603 15242603] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: hutp]] | [[Category: hutp]] | ||
[[Category: hutp-rna complex]] | [[Category: hutp-rna complex]] | ||
[[Category: national project on protein structural and functional | [[Category: national project on protein structural and functional analyse]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rna-binding]] | [[Category: rna-binding]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
[[Category: transcription/rna complex]] | [[Category: transcription/rna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:00:28 2008'' | ||
Revision as of 17:00, 20 March 2008
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| 3boy, resolution 1.70Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MG and HIS | ||||||||||||
| Gene: | hutP (Bacillus subtilis) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the HutP antitermination complex bound to the HUT mRNA
Overview
HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified.
About this Structure
3BOY is a Protein complex structure of sequences from Bacillus subtilis. This structure supersedes the now removed PDB entry 2GZT. Full crystallographic information is available from OCA.
Reference
Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603
Page seeded by OCA on Thu Mar 20 19:00:28 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bacillus subtilis
- Protein complex
- Balasundaresan, D.
- Jeyakanthan, J.
- Kumar, P K.R.
- Kumarevel, T S.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shinkai, A.
- Yokoyama, S.
- HIS
- MG
- Activator
- Anti-termination
- Histidine metabolism
- Hutp
- Hutp-rna complex
- National project on protein structural and functional analyse
- Nppsfa
- Riken structural genomics/proteomics initiative
- Rna-binding
- Rsgi
- Structural genomic
- Transcription regulation
- Transcription/rna complex
