2bo4: Difference between revisions
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==Overview== | ==Overview== | ||
The enzymatic transfer of activated mannose yields mannosides in, glycoconjugates and oligo- and polysaccharides. Yet, despite its, biological necessity, the mechanism by which glycosyltransferases, recognize mannose and catalyze its transfer to acceptor molecules is, poorly understood. Here, we report broad high-throughput screening and, kinetic analyses of both natural and synthetic substrates of Rhodothermus, marinus mannosylglycerate synthase (MGS), which catalyzes the formation of, the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS, indicates that it is at the cusp of inverting and retaining transferases., The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and, catalytic .. | The enzymatic transfer of activated mannose yields mannosides in, glycoconjugates and oligo- and polysaccharides. Yet, despite its, biological necessity, the mechanism by which glycosyltransferases, recognize mannose and catalyze its transfer to acceptor molecules is, poorly understood. Here, we report broad high-throughput screening and, kinetic analyses of both natural and synthetic substrates of Rhodothermus, marinus mannosylglycerate synthase (MGS), which catalyzes the formation of, the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS, indicates that it is at the cusp of inverting and retaining transferases., The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and, catalytic sites, unveil the mannosyl transfer center. Nucleotide, specificity is as important in GDP-D-mannose recognition as the nature of, the donor sugar. | ||
==About this Structure== | ==About this Structure== | ||
2BO4 is a | 2BO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodothermus_marinus Rhodothermus marinus] with FLC as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BO4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:25:54 2007'' | ||
