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{{STRUCTURE_4irb|  PDB=4irb  |  SCENE=  }}
==Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase Mutant del171-172D4==
===Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase Mutant del171-172D4===
<StructureSection load='4irb' size='340' side='right' caption='[[4irb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
{{ABSTRACT_PUBMED_23519808}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4irb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vacca Vacca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IRB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2owq|2owq]], [[2owr|2owr]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACAM3000_MVA_101, CPXV_GER91_3_113, D4; UNG, MVA101R, UNG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=126794 VACCA])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4irb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4irb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4irb RCSB], [http://www.ebi.ac.uk/pdbsum/4irb PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/UNG_VACCA UNG_VACCA]] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Amino-acid residues located at a highly flexible area in the uracil DNA glycosylase of Vaccinia virus were mutated. In the crystal structure of wild-type D4 these residues lie at the dimer interface. Specifically, three mutants were generated: (i) residue Arg167 was replaced with an alanine (R167AD4), (ii) residues Glu171, Ser172 and Pro173 were substituted with three glycine residues (3GD4) and (iii) residues Glu171 and Ser172 were deleted (Delta171-172D4). Mutant proteins were expressed, purified and crystallized in order to investigate the effects of these mutations on the structure of the protein.


==Function==
Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase.,Sartmatova D, Nash T, Schormann N, Nuth M, Ricciardi R, Banerjee S, Chattopadhyay D Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):295-301., doi: 10.1107/S1744309113002716. Epub 2013 Feb 23. PMID:23519808<ref>PMID:23519808</ref>
[[http://www.uniprot.org/uniprot/UNG_VACCA UNG_VACCA]] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA.  


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4irb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRB OCA].
</div>


==Reference==
==See Also==
<ref group="xtra">PMID:023519808</ref><references group="xtra"/><references/>
*[[Uracil-DNA glycosylase|Uracil-DNA glycosylase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Uracil-DNA glycosylase]]
[[Category: Uracil-DNA glycosylase]]
[[Category: Chattopadhyay, D.]]
[[Category: Vacca]]
[[Category: Nuth, M.]]
[[Category: Chattopadhyay, D]]
[[Category: Ricciardi, R P.]]
[[Category: Nuth, M]]
[[Category: Sartmatova, D.]]
[[Category: Ricciardi, R P]]
[[Category: Schormann, N.]]
[[Category: Sartmatova, D]]
[[Category: Zhukovskaya, N.]]
[[Category: Schormann, N]]
[[Category: Zhukovskaya, N]]
[[Category: Beta- sheets at n- and c-terminus]]
[[Category: Beta- sheets at n- and c-terminus]]
[[Category: Binding partners a20 and dna]]
[[Category: Binding partners a20 and dna]]

Revision as of 15:56, 24 December 2014

Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase Mutant del171-172D4

4irb, resolution 2.30Å

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