Helicase: Difference between revisions
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<StructureSection load='1pjr' size='450' side='right' scene='' caption='DNA-dependent helicase PcrA (PDB code [[1pjr]])'> | <StructureSection load='1pjr' size='450' side='right' scene='' caption='DNA-dependent helicase PcrA (PDB code [[1pjr]])'> | ||
'''Helicase''' (Hel) is a motor protein which separates nucleic acid strands like DNA double helix or self-annealed RNA. They use ATP hydrolysis for energy. Hel falls into 5 superfamilies (SF1-SF5). Some Hel contain a Helicase and RNase D C terminal | '''Helicase''' (Hel) is a motor protein which separates nucleic acid strands like DNA double helix or self-annealed RNA. They use ATP hydrolysis for energy. Hel falls into 5 superfamilies (SF1-SF5). Some Hel contain a Helicase and RNase D C terminal | ||
Domain (HRDC). The α-thalassemia and mental retardation X-linked syndrome helicase (ATRX ), contains an ATRX-Dnmt3-Dnmt3L (ADD) domain in which many disease-related mutations are found. | Domain (HRDC). The α-thalassemia and mental retardation X-linked syndrome helicase (ATRX ), contains an ATRX-Dnmt3-Dnmt3L (ADD) domain in which many disease-related mutations are found. | ||
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See also<br /> | See also<br /> | ||
*[[Transcription and RNA Processing]] | *[[Transcription and RNA Processing]] | ||
*[[Brr2]] | *[[Brr2]] - pre-mRNA-splicing helicase. | ||
== What is a Helicase? == | == What is a Helicase? == | ||
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{{clear}} | {{clear}} | ||
PcrA is part of the replication machinery of the [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]a gram (+) bacteria, This helicase is part of the superfamily I of Helicases. Monomeric protein that is mainly <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_1/Initial/1'>alpha helical</scene> has the <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_2/1pjrconser/2'>highly conserved</scene> Rec domians. This helicase was reported as a mutation in the gen PcrA from [http://en.wikipedia.org/wiki/staphylococcu "Stapphylococcus aerous"], this mutation was related to a deficiency in the replication of a reporter plasmid.[http://www.ncbi.nlm.nih.gov/pubmed/8232203?ordinalpos=81&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] | PcrA is part of the replication machinery of the [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]a gram (+) bacteria, This helicase is part of the superfamily I of Helicases. Monomeric protein that is mainly <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_1/Initial/1'>alpha helical</scene> has the <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_2/1pjrconser/2'>highly conserved</scene> Rec domians. This helicase was reported as a mutation in the gen PcrA from [http://en.wikipedia.org/wiki/staphylococcu "Stapphylococcus aerous"], this mutation was related to a deficiency in the replication of a reporter plasmid.[http://www.ncbi.nlm.nih.gov/pubmed/8232203?ordinalpos=81&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] | ||
<table align='right'><tr><td> </td><td>{{Template:ColorKey_ConSurf}}</td></tr></table> | <table align='right'><tr><td> </td><td>{{Template:ColorKey_ConSurf}}</td></tr></table> | ||
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[[Image:Mexicanwave.jpg|thumb|170px|left|Inchworm or Mexicanwave model]] | [[Image:Mexicanwave.jpg|thumb|170px|left|Inchworm or Mexicanwave model]] | ||
[[Image:Snapshot_2008-12-03_14-11-31.jpg|thumb|400px|center|PcrA Movie]] | [[Image:Snapshot_2008-12-03_14-11-31.jpg|thumb|400px|center|PcrA Movie]] | ||
'''The link below show a movie with the principal characteristics of this protain as long with the inchworm mode'''. [http://www.youtube.com/watch?v=fDwaWCkhgZI Pcr4 Helicase and Mexican Wave] | '''The link below show a movie with the principal characteristics of this protain as long with the inchworm mode'''. [http://www.youtube.com/watch?v=fDwaWCkhgZI Pcr4 Helicase and Mexican Wave] | ||
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[[4a4k]] – ySKI2 insertion domain<br /> | [[4a4k]] – ySKI2 insertion domain<br /> | ||
[[4a4z]] – ySKI2 + AMPPNP | [[4a4z]] – ySKI2 + AMPPNP | ||
===Helicase XPD=== | |||
[[3crv]], [[3crw]] – XPD – ''Sulfolobus acidocaldarius''<br /> | [[3crv]], [[3crw]] – XPD – ''Sulfolobus acidocaldarius''<br /> | ||
[[3hib]], [[3im1]], [[3im2]] – yBrr2 2nd SEC63 domain | |||
===Pre-mRNA-splicing Helicase (Brr2)=== | |||
[[3hib]], [[3im1]], [[3im2]] – yBrr2 2nd SEC63 domain<br /> | |||
[[4bgd]] – yBrr2 + pre-mRNA-splicing factor 8<br /> | |||
==References== | ==References== | ||
