5bir: Difference between revisions

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[[Image:5bir.gif|left|200px]]<br /><applet load="5bir" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:5bir.gif|left|200px]]
caption="5bir, resolution 2.00&Aring;" />
 
'''DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 USING ASN AND GLN MUTATIONS'''<br />
{{Structure
|PDB= 5bir |SIZE=350|CAPTION= <scene name='initialview01'>5bir</scene>, resolution 2.00&Aring;
|SITE= <scene name='pdbsite=CAT:Catalytic+Site'>CAT</scene>
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=2GP:GUANOSINE-2'-MONOPHOSPHATE'>2GP</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]
|GENE= SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 Aspergillus oryzae])
}}
 
'''DISECTING HISTIDINE INTERACTIONS IN RIBONUCLEASE T1 USING ASN AND GLN MUTATIONS'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
5BIR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=2GP:'>2GP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic+Site'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BIR OCA].  
5BIR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BIR OCA].  


==Reference==
==Reference==
Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position., De Vos S, Doumen J, Langhorst U, Steyaert J, J Mol Biol. 1998 Jan 30;275(4):651-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466938 9466938]
Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position., De Vos S, Doumen J, Langhorst U, Steyaert J, J Mol Biol. 1998 Jan 30;275(4):651-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9466938 9466938]
[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
[[Category: Ribonuclease T(1)]]
[[Category: Ribonuclease T(1)]]
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[[Category: ribonuclease t1]]
[[Category: ribonuclease t1]]


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