4okq: Difference between revisions

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~~{{STRUCTURE_4okq~~| ~~PDB~~=4okq | ~~SCENE~~= }}

==Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans==

===~~Crystal~~ structure of ~~the~~ single-stranded RNA ~~binding protein~~ HutP from Geobacillus thermodenitrificans~~===~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4okq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OKQ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ok9|4ok9]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTNG_0361, hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4okq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4okq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4okq RCSB], [http://www.ebi.ac.uk/pdbsum/4okq PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

RNA binding proteins control gene expression by the attenuation/antitermination mechanism. HutP is an RNA binding antitermination protein. It regulates the expression of hut operon when it binds with RNA by modulating the secondary structure of single-stranded hut mRNA. HutP necessitates the presence of l-histidine and divalent metal ion to bind with RNA. Herein, we report the crystal structures of ternary complex (HutP-l-histidine-Mg(2+)) and EDTA (0.5 M) treated ternary complex (HutP-l-histidine-Mg(2+)), solved at 1.9 A and 2.5 A resolutions, respectively, from Geobacillus thermodenitrificans. The addition of 0.5 M EDTA does not affect the overall metal-ion mediated ternary complex structure and however, the metal ions at the non-specific binding sites are chelated, as evidenced from the results of structural features.

~~==Function==~~

Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans.,Thiruselvam V, Sivaraman P, Kumarevel T, Ponnuswamy MN Biochem Biophys Res Commun. 2014 Apr 18;446(4):945-51. doi:, 10.1016/j.bbrc.2014.03.036. Epub 2014 Mar 17. PMID:24650662<ref>PMID:24650662</ref>

~~[[http~~:/~~/www~~.~~uniprot~~.~~org/uniprot~~/HUTP_GEOTN HUTP_GEOTN]] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization (By similarity).

==~~About this Structure~~==

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[~~4okq~~]] ~~is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKQ OCA].~~

</div>

[[Category: Kumarevel, T S.]]

== References ==

[[Category: Ponnuswamy, M N.]]

[[Category: Thiruselvam, V.]]

__TOC__

</StructureSection>

[[Category: Geotn]]

[[Category: Kumarevel, T S]]

[[Category: Ponnuswamy, M N]]

[[Category: Thiruselvam, V]]

[[Category: Antitermination]]

[[Category: Rna binding protein]]

[[Category: Single stranded-rna binding protein]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_4okq|  PDB=4okq  |  SCENE=  }}
+==Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans==
-===Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans===
+<StructureSection load='4okq' size='340' side='right' caption='[[4okq]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4okq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OKQ FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ok9|4ok9]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTNG_0361, hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4okq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4okq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4okq RCSB], [http://www.ebi.ac.uk/pdbsum/4okq PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+RNA binding proteins control gene expression by the attenuation/antitermination mechanism. HutP is an RNA binding antitermination protein. It regulates the expression of hut operon when it binds with RNA by modulating the secondary structure of single-stranded hut mRNA. HutP necessitates the presence of l-histidine and divalent metal ion to bind with RNA. Herein, we report the crystal structures of ternary complex (HutP-l-histidine-Mg(2+)) and EDTA (0.5 M) treated ternary complex (HutP-l-histidine-Mg(2+)), solved at 1.9 A and 2.5 A resolutions, respectively, from Geobacillus thermodenitrificans. The addition of 0.5 M EDTA does not affect the overall metal-ion mediated ternary complex structure and however, the metal ions at the non-specific binding sites are chelated, as evidenced from the results of structural features.
-==Function==
+Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans.,Thiruselvam V, Sivaraman P, Kumarevel T, Ponnuswamy MN Biochem Biophys Res Commun. 2014 Apr 18;446(4):945-51. doi:, 10.1016/j.bbrc.2014.03.036. Epub 2014 Mar 17. PMID:24650662<ref>PMID:24650662</ref>
-[[http://www.uniprot.org/uniprot/HUTP_GEOTN HUTP_GEOTN]] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization (By similarity).
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4okq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKQ OCA].
+</div>
-[[Category: Kumarevel, T S.]]
+== References ==
-[[Category: Ponnuswamy, M N.]]
+<references/>
-[[Category: Thiruselvam, V.]]
+__TOC__
+</StructureSection>
+[[Category: Geotn]]
+[[Category: Kumarevel, T S]]
+[[Category: Ponnuswamy, M N]]
+[[Category: Thiruselvam, V]]
 [[Category: Antitermination]]
 [[Category: Rna binding protein]]
 [[Category: Single stranded-rna binding protein]]