Sandbox Reserved 190: Difference between revisions
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This is a default text for your page '''Sandbox Reserved 190''' | This is a default text for your page '''Sandbox Reserved 190''' | ||
==Inhibitors of PPT-1== | |||
'''<scene name='43/436866/Overall-3-rainbow/1'>PPT-1</scene>''' is a lysosomal enzyme which had a serine lipase consensus sequence; a key characteristic of lysosomal enzymes. Despite having a serine lipase consensus sequence, PPT-1, is not deactivated by phenylmethylsulfonyl fluoride (PMSF), a common serine-modifying reagent. <scene name='58/580839/Hdsf_by_itself/1'>Hexadecylsulfonylfluoride</scene> (HDSF) is a serine-modifying reagent that is able to inhibit the actions of PPT1 <scene name='58/580839/Basic-hdsf-nosurface/4'>by binding to PPT-1</scene>. Unlike other inhibitors, <scene name='58/580839/Basic-hdsf-surfacelook/2'>HDSF is able to fit in the narrow, hydrophobic groove of PPT-1</scene> leading away from the active site of PPT-1. PMSF is unable to fit into this small narrow groove due to steric constraints that relate to the unique structure of the substrate-binding site of PPT1. The sulphur of HDSF will <scene name='58/580839/Hdsf_bound_to_Ser-115-best/1'>bind to SER-115 in the active site of PPT-1</scene> via a sulponylation reaction and thus will inhibit the actions of PPT-1. | |||
<scene name='58/580839/Hdsf_by_itself/1'>HDSF</scene> | <scene name='58/580839/Hdsf_by_itself/1'>HDSF</scene> | ||
<scene name='58/580839/Basic-hdsf-nosurface/4'> | <scene name='58/580839/Basic-hdsf-nosurface/4'>by binding to PPT-1</scene> | ||
<scene name='58/580839/Basic-hdsf-surfacelook/2'>HDSF | <scene name='58/580839/Basic-hdsf-surfacelook/2'>HDSF is able to fit in the narrow, hydrophobic groove of PPT-1</scene> | ||
<scene name='58/580839/Hdsf_bound_to_ser-115/1'> | <scene name='58/580839/Hdsf_bound_to_ser-115/1'>binds to SER-115 in the active site of PPT-1</scene> | ||
<scene name='58/580839/Hdsf_bound_to_ser-115-best/1'> | <scene name='58/580839/Hdsf_bound_to_ser-115-best/1'>bind to SER-115 in the active site of PPT-1</scene> | ||
<scene name='58/580839/Hdsf_with_ser/2'>HDSF with Ser-115</scene> | <scene name='58/580839/Hdsf_with_ser/2'>HDSF with Ser-115</scene> | ||
Revision as of 19:11, 2 April 2014
PPT-1
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