Sandbox Reserved 911: Difference between revisions
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Mutagenesis and inhibitor studies have shown that FAAH has a <scene name='57/573125/2vya/6'>Ser-Ser-Lys catalytic triad</scene>, consisting of Ser241, Ser217, and Lys142. Ser-Ser-Lys catalytic triads are not often seen in hydrolases, making FAAH an enzyme of interest for additional research to better determine how proteins with this catalytic triad function. Ser241 acts as the catalytic nucleophile for the cleavage of amide bonds. (IMT5) Inhibitors are able to inactivate the catalytic triad by providing a substrate containing a leaving group, such as aniline, that is a more favorable leaving group than the Ser241 hydroxyl group. With the serine bound to the carbonyl carbon, FAAH is no longer able to accommodate any more substrates (2VYA). FAAH also requires two water molecules in its active site to properly position and cleave amide bonds. One water molecule (W1) deacylates the substrate, and the other (W2) helps coordinate W1 through the catalytic K142. (3LJ6) | Mutagenesis and inhibitor studies have shown that FAAH has a <scene name='57/573125/2vya/6'>Ser-Ser-Lys catalytic triad</scene>, consisting of Ser241, Ser217, and Lys142. Ser-Ser-Lys catalytic triads are not often seen in hydrolases, making FAAH an enzyme of interest for additional research to better determine how proteins with this catalytic triad function. Ser241 acts as the catalytic nucleophile for the cleavage of amide bonds. (IMT5) Inhibitors are able to inactivate the catalytic triad by providing a substrate containing a leaving group, such as aniline, that is a more favorable leaving group than the Ser241 hydroxyl group. With the serine bound to the carbonyl carbon, FAAH is no longer able to accommodate any more substrates (2VYA). FAAH also requires two water molecules in its active site to properly position and cleave amide bonds. One water molecule (W1) deacylates the substrate, and the other (W2) helps coordinate W1 through the catalytic K142. (3LJ6) | ||
[[Image:Catalytic_triad2.png|1000px|left|thumb|Catalytic Triad]] | [[Image:Catalytic_triad2.png|1000px|left|thumb|Catalytic Triad Inhibited]] | ||
==Relationship to other proteins== | ==Relationship to other proteins== | ||
