4nag: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Xanthomonins I III are a New Class of Lasso Peptides Featuringa Seven-Membered Macrolactam Ring==

== Structural highlights ==

[[4nag]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAG OCA].

Activity: [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2]

== Publication Abstract from PubMed ==

Lasso peptides belong to the class of ribosomally synthesized and post-translationally modified peptides. Their common distinguishing feature is an N-terminal macrolactam ring that is threaded by the C-terminal tail. This lasso fold is maintained through steric interactions. The isolation and characterization of xanthomonins I-III, the first lasso peptides featuring macrolactam rings consisting of only seven amino acids, is now presented. The crystal structure of xanthomonin I and the NMR structure of xanthomonin II were also determined. A total of 25 variants of xanthomonin II were generated to probe different aspects of the biosynthesis, stability, and fold maintenance. These mutational studies reveal the limits such a small ring imposes on the threading and show that every plug amino acid larger than serine is able to maintain a heat-stable lasso fold in the xanthomonin II scaffold.

~~The entry 4nag is ON HOLD~~

Xanthomonins I-III: A New Class of Lasso Peptides with a Seven-Residue Macrolactam Ring.,Hegemann JD, Zimmermann M, Zhu S, Steuber H, Harms K, Xie X, Marahiel MA Angew Chem Int Ed Engl. 2014 Jan 20. doi: 10.1002/anie.201309267. PMID:24446383<ref>PMID:24446383</ref>

~~Authors~~: Hegemann, J.D.~~, Zimmermann~~, M.~~, Zhu, S.,~~ Steuber, H.~~, Harms, K.,~~ Xie, X., ~~Marahiel~~, M.A.

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.

== References ==

~~Description~~: ~~Xanthomonins I III are a New Class of~~ Lasso ~~Peptides Featuringa Seven-Membered Macrolactam Ring~~

__TOC__

</StructureSection>

[[Category: Harms, K.]]

[[Category: Hegemann, J D.]]

[[Category: Marahiel, M A.]]

[[Category: Steuber, H.]]

[[Category: Xie, X.]]

[[Category: Zhu, S.]]

[[Category: Zimmermann, M.]]

[[Category: Biosynthesis]]

[[Category: Biosynthetic protein]]

[[Category: Lariat knot]]

[[Category: Lasso fold]]

[[Category: Lasso peptide]]

[[Category: Macrocycle]]

[[Category: Natural product]]

[[Category: Steric hindrance]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Xanthomonins I III are a New Class of Lasso Peptides Featuringa Seven-Membered Macrolactam Ring==
+<StructureSection load='4nag' size='340' side='right' caption='[[4nag]], [[Resolution|resolution]] 0.81&Aring;' scene=''>
+== Structural highlights ==
+[[4nag]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAG OCA]. <br>
+<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
+== Publication Abstract from PubMed ==
+Lasso peptides belong to the class of ribosomally synthesized and post-translationally modified peptides. Their common distinguishing feature is an N-terminal macrolactam ring that is threaded by the C-terminal tail. This lasso fold is maintained through steric interactions. The isolation and characterization of xanthomonins I-III, the first lasso peptides featuring macrolactam rings consisting of only seven amino acids, is now presented. The crystal structure of xanthomonin I and the NMR structure of xanthomonin II were also determined. A total of 25 variants of xanthomonin II were generated to probe different aspects of the biosynthesis, stability, and fold maintenance. These mutational studies reveal the limits such a small ring imposes on the threading and show that every plug amino acid larger than serine is able to maintain a heat-stable lasso fold in the xanthomonin II scaffold.
-The entry 4nag is ON HOLD
+Xanthomonins I-III: A New Class of Lasso Peptides with a Seven-Residue Macrolactam Ring.,Hegemann JD, Zimmermann M, Zhu S, Steuber H, Harms K, Xie X, Marahiel MA Angew Chem Int Ed Engl. 2014 Jan 20. doi: 10.1002/anie.201309267. PMID:24446383<ref>PMID:24446383</ref>
-Authors: Hegemann, J.D., Zimmermann, M., Zhu, S., Steuber, H., Harms, K., Xie, X., Marahiel, M.A.
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+== References ==
-Description: Xanthomonins I III are a New Class of Lasso Peptides Featuringa Seven-Membered Macrolactam Ring
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Harms, K.]]
+[[Category: Hegemann, J D.]]
+[[Category: Marahiel, M A.]]
+[[Category: Steuber, H.]]
+[[Category: Xie, X.]]
+[[Category: Zhu, S.]]
+[[Category: Zimmermann, M.]]
+[[Category: Biosynthesis]]
+[[Category: Biosynthetic protein]]
+[[Category: Lariat knot]]
+[[Category: Lasso fold]]
+[[Category: Lasso peptide]]
+[[Category: Macrocycle]]
+[[Category: Natural product]]
+[[Category: Steric hindrance]]