4psm: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Crystal structure of pfuThermo-DBP-RP1 (crystal form II)==

== Structural highlights ==

[[4psm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PSM OCA].

Related: [[4psl|4psl]], [[4psn|4psn]], [[4pso|4pso]]

Activity: [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2]

== Publication Abstract from PubMed ==

Instead of a classical single-stranded deoxyribonuleic acid (DNA)-binding protein (SSB), some hyperthermophilic crenarchaea harbor a non-canonical SSB termed ThermoDBP. Two related but poorly characterized groups of proteins, which share the ThermoDBP N-terminal DNA-binding domain, have a broader phylogenetic distribution and co-exist with ThermoDBPs and/or other SSBs. We have investigated the nucleic acid binding properties and crystal structures of representatives of these groups of ThermoDBP-related proteins (ThermoDBP-RPs) 1 and 2. ThermoDBP-RP 1 and 2 oligomerize by different mechanisms and only ThermoDBP-RP2 exhibits strong single-stranded DNA affinity in vitro. A crystal structure of ThermoDBP-RP2 in complex with DNA reveals how the NTD common to ThermoDBPs and ThermoDBP-RPs can contact the nucleic acid in a manner that allows a symmetric homotetrameric protein complex to bind single-stranded DNA molecules asymmetrically. While single-stranded DNA wraps around the surface or binds along channels of previously investigated SSBs, it traverses an internal, intersubunit tunnel system of a ThermoDBP-RP2 tetramer. Our results indicate that some archaea have acquired special SSBs for genome maintenance in particularly challenging environments.

~~The entry 4psm is ON HOLD until Paper Publication~~

Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.,Ghalei H, Moeller HV, Eppers D, Sohmen D, Wilson DN, Loll B, Wahl MC Nucleic Acids Res. 2014 Apr 17. PMID:24744237<ref>PMID:24744237</ref>

~~Authors: Gahlei~~, H.~~, von Moeller, H~~., Eppers, D., Loll, B., Wahl, M.C.

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.

== References ==

~~Description~~: ~~ssDNA~~ binding protein

__TOC__

</StructureSection>

[[Category: Eppers, D.]]

[[Category: Gahlei, H.]]

[[Category: Loll, B.]]

[[Category: Moeller, H von.]]

[[Category: Wahl, M C.]]

[[Category: Dna binding protein]]

[[Category: Ssdna binding protein]]

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-'''Unreleased structure'''
+==Crystal structure of pfuThermo-DBP-RP1 (crystal form II)==
+<StructureSection load='4psm' size='340' side='right' caption='[[4psm]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
+== Structural highlights ==
+[[4psm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PSM OCA]. <br>
+<b>Related:</b> [[4psl|4psl]], [[4psn|4psn]], [[4pso|4pso]]<br>
+<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
+== Publication Abstract from PubMed ==
+Instead of a classical single-stranded deoxyribonuleic acid (DNA)-binding protein (SSB), some hyperthermophilic crenarchaea harbor a non-canonical SSB termed ThermoDBP. Two related but poorly characterized groups of proteins, which share the ThermoDBP N-terminal DNA-binding domain, have a broader phylogenetic distribution and co-exist with ThermoDBPs and/or other SSBs. We have investigated the nucleic acid binding properties and crystal structures of representatives of these groups of ThermoDBP-related proteins (ThermoDBP-RPs) 1 and 2. ThermoDBP-RP 1 and 2 oligomerize by different mechanisms and only ThermoDBP-RP2 exhibits strong single-stranded DNA affinity in vitro. A crystal structure of ThermoDBP-RP2 in complex with DNA reveals how the NTD common to ThermoDBPs and ThermoDBP-RPs can contact the nucleic acid in a manner that allows a symmetric homotetrameric protein complex to bind single-stranded DNA molecules asymmetrically. While single-stranded DNA wraps around the surface or binds along channels of previously investigated SSBs, it traverses an internal, intersubunit tunnel system of a ThermoDBP-RP2 tetramer. Our results indicate that some archaea have acquired special SSBs for genome maintenance in particularly challenging environments.
-The entry 4psm is ON HOLD  until Paper Publication
+Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.,Ghalei H, Moeller HV, Eppers D, Sohmen D, Wilson DN, Loll B, Wahl MC Nucleic Acids Res. 2014 Apr 17. PMID:24744237<ref>PMID:24744237</ref>
-Authors: Gahlei, H., von Moeller, H., Eppers, D., Loll, B., Wahl, M.C.
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+== References ==
-Description: ssDNA binding protein
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Eppers, D.]]
+[[Category: Gahlei, H.]]
+[[Category: Loll, B.]]
+[[Category: Moeller, H von.]]
+[[Category: Wahl, M C.]]
+[[Category: Dna binding protein]]
+[[Category: Ssdna binding protein]]