3p4j: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Ultra-high resolution structure of d(CGCGCG)2 Z-DNA== | |||
=== | <StructureSection load='3p4j' size='340' side='right' caption='[[3p4j]], [[Resolution|resolution]] 0.55Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3p4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P4J FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1d48|1d48]], [[1dcg|1dcg]], [[2dcg|2dcg]], [[1i0t|1i0t]], [[1ick|1ick]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p4j RCSB], [http://www.ebi.ac.uk/pdbsum/3p4j PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of a Z-DNA hexamer duplex d(CGCGCG)(2) determined at ultra high resolution of 0.55 A and refined without restraints, displays a high degree of regularity and rigidity in its stereochemistry, in contrast to the more flexible B-DNA duplexes. The estimations of standard uncertainties of all individually refined parameters, obtained by full-matrix least-squares optimization, are comparable with values that are typical for small-molecule crystallography. The Z-DNA model generated with ultra high-resolution diffraction data can be used to revise the stereochemical restraints applied in lower resolution refinements. Detailed comparisons of the stereochemical library values with the present accurate Z-DNA parameters, shows in general a good agreement, but also reveals significant discrepancies in the description of guanine-sugar valence angles and in the geometry of the phosphate groups. | |||
High regularity of Z-DNA revealed by ultra high-resolution crystal structure at 0.55 A.,Brzezinski K, Brzuszkiewicz A, Dauter M, Kubicki M, Jaskolski M, Dauter Z Nucleic Acids Res. 2011 Aug 1;39(14):6238-48. Epub 2011 Apr 1. PMID:21459852<ref>PMID:21459852</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Brzezinski, K | == References == | ||
[[Category: Brzuszkiewicz, A | <references/> | ||
[[Category: Dauter, M | __TOC__ | ||
[[Category: Dauter, Z | </StructureSection> | ||
[[Category: Jaskolski, M | [[Category: Brzezinski, K]] | ||
[[Category: Kubicki, M | [[Category: Brzuszkiewicz, A]] | ||
[[Category: Dauter, M]] | |||
[[Category: Dauter, Z]] | |||
[[Category: Jaskolski, M]] | |||
[[Category: Kubicki, M]] | |||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Z-dna]] | [[Category: Z-dna]] | ||
