1b3p: Difference between revisions
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[[Image:1b3p.gif|left|200px]] | [[Image:1b3p.gif|left|200px]] | ||
'''5'-D(*GP*GP*AP*GP*GP*AP*T)-3'''' | {{Structure | ||
|PDB= 1b3p |SIZE=350|CAPTION= <scene name='initialview01'>1b3p</scene> | |||
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'''5'-D(*GP*GP*AP*GP*GP*AP*T)-3'''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1B3P is a [ | 1B3P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. | ||
==Reference== | ==Reference== | ||
Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:[http:// | Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10425682 10425682] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bouaziz, S.]] | [[Category: Bouaziz, S.]] | ||
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[[Category: v-shaped backbone]] | [[Category: v-shaped backbone]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:05:51 2008'' | ||
Revision as of 08:05, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
5'-D(*GP*GP*AP*GP*GP*AP*T)-3'
Overview
BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases.
About this Structure
1B3P is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Interlocked mismatch-aligned arrowhead DNA motifs., Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ, Structure. 1999 Jul 15;7(7):803-15. PMID:10425682
Page seeded by OCA on Thu Mar 20 10:05:51 2008
