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{{STRUCTURE_4oww| PDB=4oww | SCENE= }}
==Structural basis of SOSS1 in complex with a 35nt ssDNA==
===Structural basis of SOSS1 in complex with a 35nt ssDNA===
<StructureSection load='4oww' size='340' side='right' caption='[[4oww]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
{{ABSTRACT_PUBMED_24630995}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4oww]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OWW FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C1orf193,C1orf60,INTS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), LP3587,NABP2,OBFC2B,SSB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), C9orf80,HSPC043,HSPC291,INIP,SSBIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oww RCSB], [http://www.ebi.ac.uk/pdbsum/4oww PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair.


==Function==
Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.,Ren W, Chen H, Sun Q, Tang X, Lim SC, Huang J, Song H Cell Rep. 2014 Mar 27;6(6):982-91. doi: 10.1016/j.celrep.2014.02.020. Epub 2014, Mar 13. PMID:24630995<ref>PMID:24630995</ref>
[[http://www.uniprot.org/uniprot/SOSSC_HUMAN SOSSC_HUMAN]] Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.<ref>PMID:19605351</ref> <ref>PMID:19683501</ref>  [[http://www.uniprot.org/uniprot/SOSB1_HUMAN SOSB1_HUMAN]] Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.<ref>PMID:18449195</ref> <ref>PMID:19605351</ref> <ref>PMID:19683501</ref>  [[http://www.uniprot.org/uniprot/INT3_HUMAN INT3_HUMAN]] Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.<ref>PMID:19605351</ref> <ref>PMID:19683501</ref>  Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites.<ref>PMID:19605351</ref> <ref>PMID:19683501</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4oww]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OWW OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024630995</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Ren, W.]]
[[Category: Ren, W.]]
[[Category: Song, H.]]
[[Category: Song, H.]]

Revision as of 06:37, 11 June 2014

Structural basis of SOSS1 in complex with a 35nt ssDNA

4oww, resolution 2.30Å

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