4jng: Difference between revisions
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==Schmallenberg virus nucleoprotein-RNA complex== | |||
===Schmallenberg virus | <StructureSection load='4jng' size='340' side='right' caption='[[4jng]], [[Resolution|resolution]] 2.12Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jng]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Sbv Sbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JNG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JNG FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4idu|4idu]], [[4idx|4idx]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133363 SBV])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jng OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jng RCSB], [http://www.ebi.ac.uk/pdbsum/4jng PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 A resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses. | |||
Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism.,Dong H, Li P, Bottcher B, Elliott RM, Dong C RNA. 2013 Aug;19(8):1129-36. doi: 10.1261/rna.039057.113. Epub 2013 Jun 24. PMID:23798666<ref>PMID:23798666</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Nucleoprotein|Nucleoprotein]] | *[[Nucleoprotein|Nucleoprotein]] | ||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Sbv]] | [[Category: Sbv]] | ||
[[Category: Bottcher, B | [[Category: Bottcher, B]] | ||
[[Category: Dong, C J | [[Category: Dong, C J]] | ||
[[Category: Dong, H H | [[Category: Dong, H H]] | ||
[[Category: Elliott, R M | [[Category: Elliott, R M]] | ||
[[Category: Li, P | [[Category: Li, P]] | ||
[[Category: Bunyavirus]] | [[Category: Bunyavirus]] | ||
[[Category: Negative-stranded rna virus nucleoprotein]] | [[Category: Negative-stranded rna virus nucleoprotein]] | ||
[[Category: Protect genomic rna]] | [[Category: Protect genomic rna]] | ||
[[Category: Viral protein-rna complex]] | [[Category: Viral protein-rna complex]] | ||
