4ckd: Difference between revisions
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==Model of complex between the E.coli enzyme beta-galactosidase and four single chain Fv antibody domains scFv13R4.== | |||
<StructureSection load='4ckd' size='340' side='right' caption='[[4ckd]], [[Resolution|resolution]] 13.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ckd]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CKD FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ckd RCSB], [http://www.ebi.ac.uk/pdbsum/4ckd PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Binding of a single-chain Fv antibody to Escherichia coli beta-galactosidase (beta-gal) is known to stabilize the enzyme and activate several inactive point mutants, historically called antibody-mediated enzyme formation mutants. To understand the nature of this activation, we have determined by electron cryo-microscopy the structure of the complex between beta-gal and the antibody scFv13R4. Our structure localizes the scFv13R4 binding site to the crevice between domains 1 and 3 in each beta-gal subunit. The mutations that scFv13R4 counteracts are located between the antibody binding site and the active site of beta-gal, at one end of the TIM-barrel that forms domain 3 where the substrate lactose is hydrolyzed. The mode of binding suggests how scFv stabilizes both the active site of beta-gal and the tetrameric state. | |||
Molecular Mechanism of Antibody-Mediated Activation of beta-galactosidase.,Vinothkumar KR, McMullan G, Henderson R Structure. 2014 Mar 4. pii: S0969-2126(14)00039-2. doi:, 10.1016/j.str.2014.01.011. PMID:24613486<ref>PMID:24613486</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Antibody|Antibody]] | *[[Antibody|Antibody]] | ||
*[[Galactosidase|Galactosidase]] | *[[Galactosidase|Galactosidase]] | ||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Beta-galactosidase]] | [[Category: Beta-galactosidase]] | ||
[[Category: Ecoli]] | [[Category: Ecoli]] | ||
[[Category: Lk3 transgenic mice]] | [[Category: Lk3 transgenic mice]] | ||
[[Category: Henderson, R | [[Category: Henderson, R]] | ||
[[Category: McMullan, G | [[Category: McMullan, G]] | ||
[[Category: Vinothkumar, K R | [[Category: Vinothkumar, K R]] | ||
[[Category: Hydrolase-immune system complex]] | [[Category: Hydrolase-immune system complex]] | ||
Revision as of 13:57, 4 January 2015
Model of complex between the E.coli enzyme beta-galactosidase and four single chain Fv antibody domains scFv13R4.
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