2qzt: Difference between revisions
New page: left|200px<br /><applet load="2qzt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qzt, resolution 1.700Å" /> '''Crystal Structure o... |
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[[Image:2qzt.jpg|left|200px]] | [[Image:2qzt.jpg|left|200px]] | ||
'''Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti''' | {{Structure | ||
|PDB= 2qzt |SIZE=350|CAPTION= <scene name='initialview01'>2qzt</scene>, resolution 1.700Å | |||
|SITE= <scene name='pdbsite=AC1:Plm+Binding+Site+For+Residue+A+300'>AC1</scene>, <scene name='pdbsite=AC2:Plm+Binding+Site+For+Residue+B+301'>AC2</scene> and <scene name='pdbsite=AC3:Plm+Binding+Site+For+Residue+A+302'>AC3</scene> | |||
|LIGAND= <scene name='pdbligand=PLM:PALMITIC ACID'>PLM</scene> | |||
|ACTIVITY= | |||
|GENE= Sterol Carrier Protein 2 Like 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 Aedes aegypti]) | |||
}} | |||
'''Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2QZT is a [ | 2QZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZT OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http:// | Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18084051 18084051] | ||
[[Category: Aedes aegypti]] | [[Category: Aedes aegypti]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sterol carrier]] | [[Category: sterol carrier]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:31:13 2008'' | ||
Revision as of 16:31, 20 March 2008
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| 2qzt, resolution 1.700Å | |||||||||||||
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| Sites: | AC1, AC2 and AC3 | ||||||||||||
| Ligands: | PLM | ||||||||||||
| Gene: | Sterol Carrier Protein 2 Like 2 (Aedes aegypti) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti
Overview
Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
About this Structure
2QZT is a Single protein structure of sequence from Aedes aegypti. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:18084051
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