2zcg: Difference between revisions
New page: left|200px<br /><applet load="2zcg" size="350" color="white" frame="true" align="right" spinBox="true" caption="2zcg, resolution 2.22Å" /> '''Structure and inhibi... |
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'''Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum''' | {{Structure | ||
|PDB= 2zcg |SIZE=350|CAPTION= <scene name='initialview01'>2zcg</scene>, resolution 2.22Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Orotidine-5'-phosphate_decarboxylase Orotidine-5'-phosphate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.23 4.1.1.23] | |||
|GENE= | |||
}} | |||
'''Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ZCG is a [ | 2ZCG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCG OCA]. | ||
==Reference== | ==Reference== | ||
Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum., Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI, Biochemistry. 2008 Feb 28;. PMID:[http:// | Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum., Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI, Biochemistry. 2008 Feb 28;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18303855 18303855] | ||
[[Category: Orotidine-5'-phosphate decarboxylase]] | [[Category: Orotidine-5'-phosphate decarboxylase]] | ||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:46 2008'' | ||
Revision as of 16:53, 20 March 2008
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| 2zcg, resolution 2.22Å | |||||||||||||
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| Activity: | Orotidine-5'-phosphate decarboxylase, with EC number 4.1.1.23 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum
Overview
Orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum ( PfODCase, EC 4.1.1.23) has been overexpressed, purified, subjected to kinetic and biochemical analysis, and crystallized. The native enzyme is a homodimer with a subunit molecular mass of 38 kDa. The saturation curve for OMP as a substrate conformed to Michaelis-Menten kinetics with K m = 350 +/- 60 nM and V max = 2.70 +/- 0.10 micromol/min/mg protein. Inhibition patterns for nucleoside 5'-monophosphate analogues were linear competitive with respect to OMP with a decreasing potency of inhibition of PfODCase in the order: pyrazofurin 5'-monophosphate ( K i = 3.6 +/- 0.7 nM) > xanthosine 5'-monophosphate (XMP, K i = 4.4 +/- 0.7 nM) > 6-azauridine 5'-monophosphate (AzaUMP, K i = 12 +/- 3 nM) > allopurinol-3-riboside 5'-monophosphate ( K i = 240 +/- 20 nM). XMP is an approximately 150-fold more potent inhibitor of PfODCase compared with the human enzyme. The structure of PfODCase was solved in the absence of ligand and displays a classic TIM-barrel fold characteristic of the enzyme. Both the phosphate-binding loop and the betaalpha5-loop have conformational flexibility, which may be associated with substrate capture and product release along the reaction pathway.
About this Structure
2ZCG is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum., Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI, Biochemistry. 2008 Feb 28;. PMID:18303855
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