2c1z: Difference between revisions

Glycosylation is a key mechanism for orchestrating the bioactivity, metabolism and location of small molecules in living cells. In plants, a, large multigene family of glycosyltransferases is involved in these, processes, conjugating hormones, secondary metabolites, biotic and abiotic, environmental toxins, to impact directly on cellular homeostasis. The red, grape enzyme UDP-glucose:flavonoid 3-O-glycosyltransferase (VvGT1) is, responsible for the formation of anthocyanins, the health-promoting, compounds which, in planta, function as colourants determining flower and, fruit colour and are precursors for the formation of pigmented polymers in, red wine. We show that VvGT1 is active, in vitro, on a range of, flavonoids. VvGT1 is somewhat promiscuous with respect to donor sugar, specificity as dissected through full kinetics on a panel of nine sugar, donors. The three-dimensional structure of VvGT1 has also been determined, both in its 'Michaelis' complex with a UDP-glucose-derived donor and the, acceptor kaempferol and in complex with UDP and quercetin. These, structures, in tandem with kinetic dissection of activity, provide the, foundation for understanding the mechanism of these enzymes in small, molecule homeostasis.

2C1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera] with KMP and U2F as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Flavonol_3-O-glucosyltransferase Flavonol 3-O-glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.91 2.4.1.91] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C1Z OCA].  

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