2xhg: Difference between revisions
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<StructureSection load='2xhg' size='340' side='right' caption='[[2xhg]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='2xhg' size='340' side='right' caption='[[2xhg]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2xhg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_8246 Atcc 8246]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHG OCA]. <br> | <table><tr><td colspan='2'>[[2xhg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_8246 Atcc 8246]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHG FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhg RCSB], [http://www.ebi.ac.uk/pdbsum/2xhg PDBsum]</span></td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhg RCSB], [http://www.ebi.ac.uk/pdbsum/2xhg PDBsum]</span></td></tr> | </table> | ||
<table> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | |||
Tyrocidine, a macrocyclic decapeptide from Bacillus brevis, is nonribosomally assembled by a set of multimodular peptide synthetases, which condense two D-amino acids and eight L-amino acids to produce this membrane-disturbing antibiotic. D-Phenylalanine, the first amino acid incorporated into tyrocidine, is catalytically derived from enzyme-bound L-Phe by the C-terminal epimerization (E) domain of tyrocidine synthetase A (TycA). The 1.5 A resolution structure of the cofactor-independent TycA E domain reveals an intimate relationship to the condensation (C) domains of peptide synthetases. In contrast to the latter, the TycA E domain uses an enlarged bridge region to plug the active-site canyon from the acceptor side, whereas at the donor side a latch-like floor loop is suitably extended to accommodate the alphaIII helix of the preceding peptide-carrier domain. Additionally, E domains exclusively harbour a conserved glutamate residue, Glu882, that is opposite the active-site residue His743. This active-site topology implies Glu882 as a candidate acid-base catalyst, whereas His743 stabilizes in the protonated state a transient enolate intermediate of the L<-->D isomerization. | |||
Structure of the epimerization domain of tyrocidine synthetase A.,Samel SA, Czodrowski P, Essen LO Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1442-52. doi:, 10.1107/S1399004714004398. Epub 2014 Apr 30. PMID:24816112<ref>PMID:24816112</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
