2c2f: Difference between revisions
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==Overview== | ==Overview== | ||
The crystal structure of a DNA-binding protein from starved cells (Dps), (DR2263) from Deinococcus radiodurans was determined in two states: a, native form, to 1.1-A resolution, and one soaked in an iron solution, to, 1.6-A resolution. In comparison with other Dps proteins, DR2263 has an, extended N-terminal extension, in both structures presented here, a novel, metal binding site was identified in this N-terminal extension and was, assigned to bound zinc. The zinc is tetrahedrally coordinated and the, ligands, that belong to the N-terminal extension, are two histidines, one, glutamate and one aspartate residue, which are unique to this protein, within the Dps family. In the iron-soaked crystal structure, a total of, three iron sites per monomer were found: one site corresponds to the, . | The crystal structure of a DNA-binding protein from starved cells (Dps), (DR2263) from Deinococcus radiodurans was determined in two states: a, native form, to 1.1-A resolution, and one soaked in an iron solution, to, 1.6-A resolution. In comparison with other Dps proteins, DR2263 has an, extended N-terminal extension, in both structures presented here, a novel, metal binding site was identified in this N-terminal extension and was, assigned to bound zinc. The zinc is tetrahedrally coordinated and the, ligands, that belong to the N-terminal extension, are two histidines, one, glutamate and one aspartate residue, which are unique to this protein, within the Dps family. In the iron-soaked crystal structure, a total of, three iron sites per monomer were found: one site corresponds to the, ferroxidase centre with structural similarities to those found in other, Dps family members; the two other sites are located on the two different, threefold axes corresponding to small pores in the Dps sphere, which may, possibly form the entrance and exit channels for iron storage. | ||
==About this Structure== | ==About this Structure== | ||
2C2F is a | 2C2F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with FE, ZN, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C2F OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: iron]] | [[Category: iron]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:47:31 2007'' | ||
