Sandbox Reserved 935: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
|||
| Line 28: | Line 28: | ||
BRI1 belongs to a group of membrane receptor kinases called LRR-RKs with an extracellular LRR ligand binding domain, a single membrane spanning helix and a cytoplasmic kinase domain <ref name=Bojar2014 />. The BRI1 nucleotide binding site is located between the N- and C-lobes of the protein. | BRI1 belongs to a group of membrane receptor kinases called LRR-RKs with an extracellular LRR ligand binding domain, a single membrane spanning helix and a cytoplasmic kinase domain <ref name=Bojar2014 />. The BRI1 nucleotide binding site is located between the N- and C-lobes of the protein. | ||
The kinase domain adopts an active conformation with a salt-bridge between Lys911 and Glu927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glu927 and Tyr956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation. | The kinase domain adopts an active conformation with a salt-bridge between Lys911 and Glu927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glu927 and Tyr956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation, because they are holding the binding pocket in its active conformation. | ||
==Function== | ==Function== | ||
