Talk:Resolution: Difference between revisions

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Eric Martz (talk | contribs)
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Eric Martz (talk | contribs)
ConfirmAccount, Editor, Print3D, Tester, Bureaucrats, journal, Administrators
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Proposed revision (May, 2014) after discussion with Keiichi Namba. Comments welcome!
Proposed revision (May, 2014) after discussion with Keiichi Namba. Comments welcome!


Resolution of a macromolecular crystal is a value that quantitates the uncertainty of atomic positions in the portion of the macromolecule that is most ordered in the crystal. High numeric values (such as 4 Å) mean high uncertainty, "poor resolution"; low values (such as 1.5 Å) mean low uncertainty, "good resolution". The most-ordered portion of the macromolecule is responsible for diffraction spots that are farthest from the axis of the X-ray beam. Resolution is determined from these farthest spots based on their angle  θ from the X-ray beam, using [http://en.wikipedia.org/wiki/Bragg's_law the Bragg equation]:
The resolution of a macromolecular crystal is the smallest distance between crystal lattice planes that is resolved in the diffraction pattern. Thus high numeric values of resolution, such as 4 Å, mean poor resolution, while low numeric values, such as 1.5 Å, mean good resolution.
 
The most-ordered portion of the macromolecule is responsible for diffraction spots that are farthest from the axis of the X-ray beam. Resolution is determined from these farthest spots based on their angle  θ from the X-ray beam, using [http://en.wikipedia.org/wiki/Bragg's_law the Bragg equation]:
<center>
<center>
d = &lambda; / (2 sin &theta;)
d = &lambda; / (2 sin &theta;)
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