Talk:Resolution: Difference between revisions
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Eric Martz (talk | contribs) No edit summary |
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The portion of the macromolecule that is best ordered in the crystal is responsible for diffraction spots that are farthest from the axis of the X-ray beam. Resolution is determined from these farthest spots based on their angle θ from the X-ray beam, using [http://en.wikipedia.org/wiki/Bragg's_law the Bragg equation]: | The portion of the macromolecule that is best ordered in the crystal is responsible for diffraction spots that are farthest from the axis of the X-ray beam. Resolution is determined from these farthest spots based on their angle θ from the X-ray beam, using [http://en.wikipedia.org/wiki/Bragg's_law the Bragg equation] solved for ''d'': | ||
<center> | <center> | ||
d = λ / (2 sin θ) | ''d'' = λ / (2 sin θ) | ||
</center> | </center> | ||
where λ is the X-ray wavelength, and ''d'' is the smallest distance between crystal lattice planes that scatter discrete spots. The resolution is given by ''d''. | where λ is the X-ray wavelength, and ''d'' is the smallest distance between crystal lattice planes that scatter discrete spots. The resolution is given by ''d''. | ||
