Sandbox Reserved 935: Difference between revisions

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~~<scene name='57/579705/Nucleotide_binding_pocket/16'>nucleotide binding pocket</scene>~~

==Introduction==

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BRI1 belongs to a group of membrane receptor kinases called LRR-RKs with an extracellular LRR ligand binding domain, a single membrane spanning helix and a cytoplasmic kinase domain <ref name=Bojar2014 />. The BRI1 nucleotide binding site is located between the N- and C-lobes of the protein.

The kinase domain adopts an active conformation with a salt-bridge between Lysine-911 and Glutamate-927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glutamate-927 and Tyrosine-956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation, because they are holding the binding pocket in its active conformation.

The kinase domain <scene name='57/579705/Nucleotide_binding_pocket/16'>nucleotide binding pocket</scene> adopts an active conformation with a salt-bridge between Lysine-911 and Glutamate-927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glutamate-927 and Tyrosine-956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation, because they are holding the binding pocket in its active conformation.

==Function==

Sandbox Reserved 935: Difference between revisions

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 <Structure load='4OA2' size='350' frame='true' align='right' caption='Insert caption here' scene='57/579705/Testi/1' />
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-<scene name='57/579705/Nucleotide_binding_pocket/16'>nucleotide binding pocket</scene>
 ==Introduction==
@@ Line 25: / Line 23: @@
 BRI1 belongs to a group of membrane receptor kinases called LRR-RKs with an extracellular LRR ligand binding domain, a single membrane spanning helix and a cytoplasmic kinase domain <ref name=Bojar2014 />. The BRI1 nucleotide binding site is located between the N- and C-lobes of the protein.
-The kinase domain adopts an active conformation with a salt-bridge between Lysine-911 and Glutamate-927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glutamate-927 and Tyrosine-956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation, because they are holding the binding pocket in its active conformation.
+The kinase domain <scene name='57/579705/Nucleotide_binding_pocket/16'>nucleotide binding pocket</scene> adopts an active conformation with a salt-bridge between Lysine-911 and Glutamate-927 <ref name=Bojar2014 />. There is also a hydrogen bond between Glutamate-927 and Tyrosine-956. This tyrosine residue is a gatekeeper determining the size of the nucleotide binding pocket. Comparison with other plant receptor-like kinases suggests this hydrogen bond interaction and salt-bridge are important for the activation, because they are holding the binding pocket in its active conformation.
 ==Function==