Sandbox Reserved 933: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 15: | Line 15: | ||
== Structural Basis of LEAFY binding == | == Structural Basis of LEAFY binding == | ||
=== General information about the structure === | === General information about the structure === | ||
The ''LFY'' gene encodes a 424 amino acids protein that containing two domains. The N-terminal domain of LFY has been proved mediating homodimerization (ref) and it is also thought to be responsible for transcriptional activation (ref). The C-terminal consensus is highly conserved among land species and functioning as DNA-binding domain. Two DNA-protein binding structure for LEAFY were first published by Hame et al. 2008 (ref). These two structures include a recombinant C-terminal domain of LEAFY expressed by ''Escherichia coli'' strain RosettaBlue (DE3) and a short nucleotide structure from AP1 or AG promoter region. Final models of LFY-pAP1 and LFY-pAG were solved at 2.1 Å and 2.3 Å by X-ray diffraction and deposited as PDB entry <scene name='57/579703/2vy1/1'>2VY1</scene>/<scene name='57/579703/2vy2/1'>2VY2</scene>. The DNA binding domain | The ''LFY'' gene encodes a 424 amino acids protein that containing two domains. The N-terminal domain of LFY has been proved mediating homodimerization (ref) and it is also thought to be responsible for transcriptional activation (ref). The C-terminal consensus is highly conserved among land species and functioning as DNA-binding domain. Two DNA-protein binding structure for LEAFY were first published by Hame et al. 2008 (ref). These two structures include a recombinant C-terminal domain of LEAFY expressed by ''Escherichia coli'' strain RosettaBlue (DE3) and a short nucleotide structure from AP1 or AG promoter region. Final models of LFY-pAP1 and LFY-pAG were solved at 2.1 Å and 2.3 Å by X-ray diffraction and deposited as PDB entry <scene name='57/579703/2vy1/1'>2VY1</scene>/<scene name='57/579703/2vy2/1'>2VY2</scene>. | ||
=== DNA recognition is conducted by a HTH-like motif === | |||
The general structure of LEAFY DNA binding domain consists 2 <scene name='57/579703/Beta-strand/1'>β strands</scene> at the beginning followed by 7 <scene name='57/579703/Alpha-helices_color/2'> α helices</scene>. | |||
=== DNA binding required cooperative dimerization === | === DNA binding required cooperative dimerization === | ||
<scene name='57/579703/Ap1-dimer/1'>LFY-pAP1</scene> | <scene name='57/579703/Ap1-dimer/1'>LFY-pAP1</scene> | ||
