Sandbox Reserved 933: Difference between revisions
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<StructureSection load='2VY1' size='400' side= 'right' caption='Structure of LFY binding with AP1 and AG promoter region (PDB entry: 2VY1/2VY2)' Scene=57/579703/2vy1/1> | <StructureSection load='2VY1' size='400' side= 'right' caption='Structure of LFY binding with AP1 and AG promoter region (PDB entry: 2VY1/2VY2)' Scene=57/579703/2vy1/1> | ||
== Structural Basis of LEAFY binding == | == Structural Basis of LEAFY binding == | ||
[[Image:DNA_Recognition.jpg|300px|left|thumb| Figure 2. Site specific recognition of LFY protein at major (A) and minor (B) grooves. Assembly of PDB entry 2VY1 were obtained from PISA server and further visualized by Pymol. Red arrows marked site specific hydrogen bonds.]] | |||
=== General information about the structure === | === General information about the structure === | ||
The ''LFY'' gene encodes a 424 amino acids protein that containing two domains. The N-terminal domain of LFY has been proved mediating homodimerization (ref) and it is also thought to be responsible for transcriptional activation (ref). The C-terminal consensus is highly conserved among land species and functioning as DNA-binding domain. Two DNA-protein binding structure for LEAFY were first published by Hame et al. 2008 (ref). These two structures include a recombinant C-terminal domain of LEAFY expressed by ''Escherichia coli'' strain RosettaBlue (DE3) and a short nucleotide structure from AP1 or AG promoter region. Final models of LFY-pAP1 and LFY-pAG were solved at 2.1 Å and 2.3 Å by X-ray diffraction and deposited as PDB entry <scene name='57/579703/2vy1/1'>2VY1</scene>/<scene name='57/579703/2vy2/1'>2VY2</scene>. | The ''LFY'' gene encodes a 424 amino acids protein that containing two domains. The N-terminal domain of LFY has been proved mediating homodimerization (ref) and it is also thought to be responsible for transcriptional activation (ref). The C-terminal consensus is highly conserved among land species and functioning as DNA-binding domain. Two DNA-protein binding structure for LEAFY were first published by Hame et al. 2008 (ref). These two structures include a recombinant C-terminal domain of LEAFY expressed by ''Escherichia coli'' strain RosettaBlue (DE3) and a short nucleotide structure from AP1 or AG promoter region. Final models of LFY-pAP1 and LFY-pAG were solved at 2.1 Å and 2.3 Å by X-ray diffraction and deposited as PDB entry <scene name='57/579703/2vy1/1'>2VY1</scene>/<scene name='57/579703/2vy2/1'>2VY2</scene>. | ||
=== Site specific DNA recognition is conducted by a HTH-like motif === | === Site specific DNA recognition is conducted by a HTH-like motif === | ||
