4p0s

human Mus81-Eme1-3'flap DNA complex

spinqualitylabelsall models 4p0s, resolution 6.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4p0s is a 20 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 4p0p, 4p0q, 4p0r Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. To explain the molecular basis of 3' flap substrate recognition and cleavage mechanism by Mus81-Eme1, we determined crystal structures of human Mus81-Eme1 bound to various flap DNA substrates. Mus81-Eme1 undergoes gross substrate-induced conformational changes that reveal two key features: (i) a hydrophobic wedge of Mus81 that separates pre- and post-nick duplex DNA and (ii) a "5' end binding pocket" that hosts the 5' nicked end of post-nick DNA. These features are crucial for comprehensive protein-DNA interaction, sharp bending of the 3' flap DNA substrate, and incision strand placement at the active site. While Mus81-Eme1 unexpectedly shares several common features with members of the 5' flap nuclease family, the combined structural, biochemical, and biophysical analyses explain why Mus81-Eme1 preferentially cleaves 3' flap DNA substrates with 5' nicked ends. Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates.,Gwon GH, Jo A, Baek K, Jin KS, Fu Y, Lee JB, Kim Y, Cho Y EMBO J. 2014 May 2;33(9):1061-72. doi: 10.1002/embj.201487820. Epub 2014 Apr 14. PMID:24733841 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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