4if5: Difference between revisions
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4if5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4if5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4if5 RCSB], [http://www.ebi.ac.uk/pdbsum/4if5 PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4if5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4if5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4if5 RCSB], [http://www.ebi.ac.uk/pdbsum/4if5 PDBsum]</span></td></tr> | ||
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== Publication Abstract from PubMed == | |||
Tubulin protomers undergo an extensive array of post-translational modifications to tailor microtubules to specific tasks. One such modification, the acetylation of lysine 40 of alpha-tubulin, located in the lumen of microtubules, is associated with stable, long-living microtubule structures. MEC-17 was recently identified as the acetyltransferase that mediates this event. We have determined the crystal structure of the catalytic core of human MEC-17 in complex with its cofactor acetyl-CoA at 1.7A resolution. The structure reveals that the MEC-17 core adopts a canonical Gcn5-related N-acetyltransferase (GNAT) fold that is decorated with extensive surface loops. An enzymatic analysis of 33 MEC-17 surface mutants identifies hot-spot residues for catalysis and substrate recognition. A large, evolutionarily conserved hydrophobic surface patch that is critical for enzymatic activity is identified, suggesting that specificity is achieved by interactions with the alpha-tubulin substrate that extend outside of the modified surface loop. An analysis of MEC-17 mutants in Caenorhabditis elegans shows that enzymatic activity is dispensable for touch sensitivity. | |||
Structural and Functional Characterization of the alpha-Tubulin Acetyltransferase MEC-17.,Davenport AM, Collins LN, Chiu H, Minor PJ, Sternberg PW, Hoelz A J Mol Biol. 2014 Jul 15;426(14):2605-16. doi: 10.1016/j.jmb.2014.05.009. Epub, 2014 May 17. PMID:24846647<ref>PMID:24846647</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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== References == | |||
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