3udg

Structure of Deinococcus radiodurans SSB bound to ssDNA

spinqualitylabelsall models 3udg, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3udg is a 9 chain structure with sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: TMP Gene: DR_0099, ssb (Deinococcus radiodurans) Resources: FirstGlance, OCA, RCSB, PDBsum Function [SSB_DEIRA] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). Publication Abstract from PubMed The single-strand (ss) DNA-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetramers of single-OB-domain subunits. To better understand how this unusual structure influences the DNA binding and biological functions of DrSSB in D. radiodurans radiation resistance, we have examined the structure of DrSSB in complex with ssDNA and the DNA-damage-dependent cellular dynamics of DrSSB. The X-ray crystal structure of the DrSSB/ssDNA complex shows that ssDNA binds to surfaces of DrSSB that are analogous to those mapped in homotetrameric SSBs, although there are distinct contacts in DrSSB that mediate species-specific ssDNA binding. Observations by electron microscopy reveal two salt-dependent ssDNA-binding modes for DrSSB that strongly resemble those of the homotetrameric Escherichia coli SSB, further supporting a shared overall DNA binding mechanism between the two classes of bacterial SSBs. In vivo, DrSSB levels are heavily induced following exposure to ionizing radiation. This accumulation is accompanied by dramatic time-dependent DrSSB cellular dynamics in which a single nucleoid-centric focus of DrSSB is observed within an hour of irradiation but is dispersed by three hours after irradiation. These kinetics parallel those of D. radiodurans post-irradiation genome reconstitution, suggesting that DrSSB dynamics could play important organizational roles in DNA repair. Structure and cellular dynamics of Deinococcus radiodurans SSB/DNA complexes.,George NP, Ngo KV, Chitteni-Pattu S, Norais CA, Battista JR, Cox MM, Keck JL J Biol Chem. 2012 May 7. PMID:22570477 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Single-stranded DNA-binding protein

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