1nla

Solution Structure of Switch Arc, a Mutant with 3(10) Helices Replacing a Wild-Type Beta-Ribbon

spinqualitylabelsall models 1nla, 13 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1nla is a 2 chain structure with sequence from Enterobacteria phage p22. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1qtg Gene: ARC (Enterobacteria phage P22) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Adjacent N11L and L12N mutations in the antiparallel beta-ribbon of Arc repressor result in dramatic changes in local structure in which each beta-strand is replaced by a right-handed helix. The full solution structure of this "switch" Arc mutant shows that irregular 3(10) helices compose the new secondary structure. This structural metamorphosis conserves the number of main-chain and side-chain to main-chain hydrogen bonds and the number of fully buried core residues. Apart from a slight widening of the interhelical angle between alpha-helices A and B and changes in side-chain conformation of a few core residues in Arc, no large-scale structural adjustments in the remainder of the protein are necessary to accommodate the ribbon-to-helix change. Nevertheless, some changes in hydrogen-exchange rates are observed, even in regions that have very similar structures in the two proteins. The surface of switch Arc is packed poorly compared to wild-type, leading to approximately 1000A(2) of additional solvent-accessible surface area, and the N termini of the 3(10) helices make unfavorable head-to-head electrostatic interactions. These structural features account for the positive m value and salt dependence of the ribbon-to-helix transition in Arc-N11L, a variant that can adopt either the mutant or wild-type structures. The tertiary fold is capped in different ways in switch and wild-type Arc, showing how stepwise evolutionary transformations can arise through small changes in amino acid sequence. Solution structure of switch Arc, a mutant with 3(10) helices replacing a wild-type beta-ribbon.,Cordes MH, Walsh NP, McKnight CJ, Sauer RT J Mol Biol. 2003 Feb 21;326(3):899-909. PMID:12581649 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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