4qyz: Difference between revisions
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''' | ==Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target== | ||
<StructureSection load='4qyz' size='340' side='right' caption='[[4qyz]], [[Resolution|resolution]] 3.03Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4qyz]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QYZ FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qyz RCSB], [http://www.ebi.ac.uk/pdbsum/4qyz PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In prokaryotes, RNA derived from type I and type III CRISPR loci direct large ribonucleoprotein complexes to destroy invading bacteriophage and plasmids. In Escherichia coli, this 405-kDa complex is called Cascade. Here, we report the 3.03A crystal structure of Cascade bound to a single-stranded DNA target. The structure reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure. This non-canonical structure is facilitated by rotation of every sixth nucleotide out of the RNA-DNA hybrid and is stabilized by the highly interlocked organization of protein subunits. These studies provide insight into both the assembly and the activity of this complex and suggest a mechanism to enforce fidelity of target binding. | |||
Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target.,Mulepati S, Heroux A, Bailey S Science. 2014 Aug 14. pii: 1256996. PMID:25123481<ref>PMID:25123481</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bailey, S.]] | |||
[[Category: Mulepati, S.]] | |||
[[Category: Bacterial immunity]] | |||
[[Category: Cas3]] | |||
[[Category: Crispr-associated]] | |||
[[Category: Immune system-dna-rna complex]] | |||
Revision as of 05:51, 4 September 2014
Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target
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