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| ==mammalian 80S HCV-IRES initiation complex with eIF5B PRE-like state==
| | #REDIRECT [[4ujd]] This PDB entry is obsolete and replaced by 4ujd |
| <StructureSection load='4upw' size='340' side='right' caption='[[4upw]], [[Resolution|resolution]] 8.90Å' scene=''>
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| == Structural highlights ==
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| <table><tr><td colspan='2'>[[4upw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UPW FirstGlance]. <br>
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| </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4upx|4upx]], [[4upy|4upy]], [[4uq5|4uq5]], [[4upz|4upz]], [[4uq0|4uq0]], [[4uq1|4uq1]], [[4uq4|4uq4]]</td></tr>
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| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4upw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4upw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4upw RCSB], [http://www.ebi.ac.uk/pdbsum/4upw PDBsum]</span></td></tr>
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| <table>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAiMet). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAiMet-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAiMet to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.
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| Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.,Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Burger J, Mielke T, Spahn CM Nat Struct Mol Biol. 2014 Aug;21(8):721-727. doi: 10.1038/nsmb.2859. Epub 2014, Jul 27. PMID:25064512<ref>PMID:25064512</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| == References ==
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| <references/>
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| __TOC__
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| </StructureSection>
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| [[Category: Oryctolagus cuniculus]]
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| [[Category: Behrmann, E.]]
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| [[Category: Burger, J.]]
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| [[Category: Loerke, J.]]
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| [[Category: Marianne, C.]]
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| [[Category: Mielke, T.]]
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| [[Category: Spahn, C M.T.]]
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| [[Category: Unbehaun, A.]]
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| [[Category: Yamamoto, H.]]
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| [[Category: Eukaryotic initiation factor 5b]]
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| [[Category: Hepatitis c virus internal ribosome entry site]]
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| [[Category: Ribosome]]
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| [[Category: Translation initiation]]
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This PDB entry is obsolete and replaced by 4ujd
