1dm1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 1dm1 |SIZE=350|CAPTION= <scene name='initialview01'>1dm1</scene>, resolution 1.99Å | |PDB= 1dm1 |SIZE=350|CAPTION= <scene name='initialview01'>1dm1</scene>, resolution 1.99Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dm1 OCA], [http://www.ebi.ac.uk/pdbsum/1dm1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dm1 RCSB]</span> | |||
}} | }} | ||
| Line 28: | Line 31: | ||
[[Category: Savino, C.]] | [[Category: Savino, C.]] | ||
[[Category: Travaglini-Allocatelli, C.]] | [[Category: Travaglini-Allocatelli, C.]] | ||
[[Category: globin fold]] | [[Category: globin fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:44:14 2008'' | ||
Revision as of 16:44, 30 March 2008
| |||||||||||||
| 1dm1, resolution 1.99Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | HEM | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
2.0 A CRYSTAL STRUCTURE OF THE DOUBLE MUTANT H(E7)V, T(E10)R OF MYOGLOBIN FROM APLYSIA LIMACINA
Overview
Aplysia limacina myoglobin lacks the distal histidine (His (E7)) and displays a ligand stabilization mechanism based on Arg(E10). The double mutant Val(E7)His-Arg(E10)Thr has been prepared to engineer the role of His(E7), typical of mammalian myoglobins, in a different globin framework. The 2.0 A crystal structure of Val(E7)His-Arg(E10)Thr met-Mb mutant reveals that the His(E7) side chain points out of the distal pocket, providing an explanation for the observed failure to stabilize the Fe(II) bound oxygen in the ferrous myoglobin. Moreover, spectroscopic analysis together with kinetic data on azide binding to met-myoglobin are reported and discussed in terms of the presence of a water molecule at coordination distance from the heme iron.
About this Structure
1DM1 is a Single protein structure of sequence from Aplysia limacina. Full crystallographic information is available from OCA.
Reference
Engineering His(E7) affects the control of heme reactivity in Aplysia limacina myoglobin., Federici L, Savino C, Musto R, Travaglini-Allocatelli C, Cutruzzola F, Brunori M, Biochem Biophys Res Commun. 2000 Mar 5;269(1):58-63. PMID:10694477
Page seeded by OCA on Sun Mar 30 19:44:14 2008
