2cn2: Difference between revisions
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==Overview== | ==Overview== | ||
The enzymatic degradation of the plant cell wall is central both to the, natural carbon cycle and, increasingly, to environmentally friendly routes, to biomass conversion, including the production of biofuels. The plant, cell wall is a complex composite of cellulose microfibrils embedded in, diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is, one such polysaccharide whose hydrolysis is catalyzed by diverse, xyloglucanases. Here we present the structure of the Clostridium, thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms., The structures, in combination with mutagenesis data on the catalytic, residues and the kinetics and specificity of xyloglucan hydrolysis reveal, a complex subsite specificity accommodating seventeen monosaccharide, moieties . | The enzymatic degradation of the plant cell wall is central both to the, natural carbon cycle and, increasingly, to environmentally friendly routes, to biomass conversion, including the production of biofuels. The plant, cell wall is a complex composite of cellulose microfibrils embedded in, diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is, one such polysaccharide whose hydrolysis is catalyzed by diverse, xyloglucanases. Here we present the structure of the Clostridium, thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms., The structures, in combination with mutagenesis data on the catalytic, residues and the kinetics and specificity of xyloglucan hydrolysis reveal, a complex subsite specificity accommodating seventeen monosaccharide, moieties of the multibranched substrate in an open substrate binding, terrain. | ||
==About this Structure== | ==About this Structure== | ||
2CN2 is a | 2CN2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Xyloglucan-specific_endo-beta-1,4-glucanase Xyloglucan-specific endo-beta-1,4-glucanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.151 3.2.1.151] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CN2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: xyloglucanase]] | [[Category: xyloglucanase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:27:15 2007'' | ||
