2ixp: Difference between revisions
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==Overview== | ==Overview== | ||
PTPA, an essential and specific activator of protein phosphatase 2A, (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here, the crystal structures of human PTPA and of the two yeast orthologs (Ypa1, and Ypa2), revealing an all alpha-helical protein fold that is radically, different from other PPIases. The protein is organized into two domains, separated by a groove lined by highly conserved residues. To understand, the molecular mechanism of PTPA activity, Ypa1 was cocrystallized with a, proline-containing PPIase peptide substrate. In the complex, the peptide, binds at the interface of a peptide-induced dimer interface. Conserved, residues of the interdomain groove contribute to the peptide binding site, and dimer interface. Structure-guided mutational studies showed . | PTPA, an essential and specific activator of protein phosphatase 2A, (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here, the crystal structures of human PTPA and of the two yeast orthologs (Ypa1, and Ypa2), revealing an all alpha-helical protein fold that is radically, different from other PPIases. The protein is organized into two domains, separated by a groove lined by highly conserved residues. To understand, the molecular mechanism of PTPA activity, Ypa1 was cocrystallized with a, proline-containing PPIase peptide substrate. In the complex, the peptide, binds at the interface of a peptide-induced dimer interface. Conserved, residues of the interdomain groove contribute to the peptide binding site, and dimer interface. Structure-guided mutational studies showed that in, vivo PTPA activity is influenced by mutations on the surface of the, peptide binding pocket, the same mutations that also influenced the in, vitro activation of PP2Ai and PPIase activity. | ||
==About this Structure== | ==About this Structure== | ||
2IXP is a | 2IXP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4, CL and SIN as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IXP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pp2a phosphatase activator prolyl isomerase ptpa]] | [[Category: pp2a phosphatase activator prolyl isomerase ptpa]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:21:16 2007'' | ||
