1plb

HIGH-RESOLUTION SOLUTION STRUCTURE OF REDUCED PARSLEY PLASTOCYANIN

spinqualitylabelsall models 1plb, 1 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1plb is a 1 chain structure with sequence from Petroselinum crispum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU Related: 1pla Resources: FirstGlance, OCA, RCSB, PDBsum Function [PLAS_PETCR] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed A high-resolution three-dimensional solution structure of parsley plastocyanin has been determined using 1H-NMR-derived data. An ensemble of 30 conformers has been calculated, exhibiting an atomic root mean square distribution about the mean coordinate positions of 0.37 +/- 0.03 A for backbone atoms and 0.75 +/- 0.04 A for all heavy atoms. (These values exclude residues 8-10 which are disordered.) The global fold of parsley plastocyanin is closely similar to those of other plastocyanins which have been structurally characterized by X-ray diffraction and NMR methods. However, deletion of residues at positions 57 and 58 of the consensus plastocyanin sequence causes elimination of a turn found in most higher plant plastocyanins. This turn is located in an acidic patch binding site, which consists of two clusters of acidic residues at positions 42-45 and 59-61. These residues surround the side chain of Tyr 83, which has been shown to be involved in binding of and electron transfer from cytochrome f, one of plastocyanin's physiological partners. The acidic recognition site is further disrupted in parsley plastocyanin by nonconservative substitution of two charged residues at positions 59 and 60. The NMR-derived structures show that E53, E85, and E95 compensate for these substitutions and give parsley plastocyanin an acidic recognition site of similar extent to that of other higher plant plastocyanins.(ABSTRACT TRUNCATED AT 250 WORDS) High-resolution solution structure of reduced parsley plastocyanin.,Bagby S, Driscoll PC, Harvey TS, Hill HA Biochemistry. 1994 May 31;33(21):6611-22. PMID:8204598 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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