2j0s: Difference between revisions

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==Overview==

The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing ..~~. [[http://ispc.weizmann.ac.il/pmbin/getpm?16923391 (full description)]]~~

The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing the ATP bound state. Comparison with the structure of the, eIF4AIII-Btz subcomplex that we have also determined reveals that large, conformational changes are required upon EJC assembly and disassembly.

==About this Structure==

2J0S is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and ANP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0S OCA]].

2J0S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0S OCA].

==Reference==

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[[Category: transport]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on ~~Tue Oct 30 17~~:21:14 2007''

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:36:20 2007''

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@@ Line 5: / Line 5: @@
 ==Overview==
-The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16923391 (full description)]]
+The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing the ATP bound state. Comparison with the structure of the, eIF4AIII-Btz subcomplex that we have also determined reveals that large, conformational changes are required upon EJC assembly and disassembly.
 ==About this Structure==
-J0S is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and ANP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0S OCA]].
+J0S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0S OCA].
 ==Reference==
@@ Line 40: / Line 40: @@
 [[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:21:14 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 13:36:20 2007''