3i4r: Difference between revisions

Revision as of 06:57, 24 December 2014

Nup107(aa658-925)/Nup133(aa517-1156) complex, H.sapiens

Structural highlights

3i4r is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	NUP107 (Homo sapiens), NUP133 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[NU107_HUMAN] Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex.^[1] [NU133_HUMAN] Involved in poly(A)+ RNA transport.^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The nuclear pore complex (NPC) constitutes one of the largest protein assemblies in the eukaryotic cell and forms the exclusive gateway to the nucleus. The stable, approximately 15-20-MDa scaffold ring of the NPC is built from two multiprotein complexes arranged around a central 8-fold axis. Here we present crystal structures of two large architectural units, yNup170(979-1502) and hNup107(658-925) x hNup133(517-1156), each a constituent of one of the two multiprotein complexes. Conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. Together with the previously established ancestral coatomer element (ACE1), these two elements constitute the major alpha-helical building blocks of the NPC scaffold and define its branched, lattice-like architecture, similar to vesicle coats like COPII. We hypothesize that the extant NPC evolved early during eukaryotic evolution from a rudimentary structure composed of several identical copies of a few ancestral elements, later diversified and specified by gene duplication.

Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.,Whittle JR, Schwartz TU J Biol Chem. 2009 Oct 9;284(41):28442-52. Epub 2009 Aug 11. PMID:19674973^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boehmer T, Enninga J, Dales S, Blobel G, Zhong H. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):981-5. Epub 2003 Jan 27. PMID:12552102 doi:https://dx.doi.org/10.1073/pnas.252749899
↑ Vasu S, Shah S, Orjalo A, Park M, Fischer WH, Forbes DJ. Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. J Cell Biol. 2001 Oct 29;155(3):339-54. Epub 2001 Oct 29. PMID:11684705 doi:https://dx.doi.org/10.1083/jcb.200108007
↑ Whittle JR, Schwartz TU. Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element. J Biol Chem. 2009 Oct 9;284(41):28442-52. Epub 2009 Aug 11. PMID:19674973 doi:10.1074/jbc.M109.023580

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Boehmer T, Enninga J, Dales S, Blobel G, Zhong H. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):981-5. Epub 2003 Jan 27. PMID:12552102 doi:https://dx.doi.org/10.1073/pnas.252749899

[2] Vasu S, Shah S, Orjalo A, Park M, Fischer WH, Forbes DJ. Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. J Cell Biol. 2001 Oct 29;155(3):339-54. Epub 2001 Oct 29. PMID:11684705 doi:https://dx.doi.org/10.1083/jcb.200108007

[3] Whittle JR, Schwartz TU. Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element. J Biol Chem. 2009 Oct 9;284(41):28442-52. Epub 2009 Aug 11. PMID:19674973 doi:10.1074/jbc.M109.023580

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[2]

[3]

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3i4r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I4R FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NUP107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), NUP133 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NUP107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), NUP133 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i4r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i4r RCSB], [http://www.ebi.ac.uk/pdbsum/3i4r PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i4r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i4r RCSB], [http://www.ebi.ac.uk/pdbsum/3i4r PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/NU107_HUMAN NU107_HUMAN]] Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex.<ref>PMID:12552102</ref>  [[http://www.uniprot.org/uniprot/NU133_HUMAN NU133_HUMAN]] Involved in poly(A)+ RNA transport.<ref>PMID:11684705</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 34: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Schwartz, T U.]]
+[[Category: Schwartz, T U]]
-[[Category: Whittle, J R.R.]]
+[[Category: Whittle, J R.R]]
 [[Category: Kinetochore]]
 [[Category: Mrna transport]]

3i4r: Difference between revisions

Revision as of 06:57, 24 December 2014

Nup107(aa658-925)/Nup133(aa517-1156) complex, H.sapiens

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

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References

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