3egm

Structural basis of iron transport gating in Helicobacter pylori ferritin

spinqualitylabelsall models 3egm, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3egm is a 6 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE, GOL Gene: ftnA, pfr, jhp_0598 (Helicobacter pylori) Activity: Ferroxidase, with EC number 1.16.3.1 Resources: FirstGlance, OCA, RCSB, PDBsum Function [FTN_HELPJ] Iron-storage protein (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The crystal structure of recombinant ferritin from Helicobacter pylori has been determined in its apo, low-iron-bound, intermediate, and high-iron-bound states. Similar to other members of the ferritin family, the bacterial ferritin assembles as a spherical protein shell of 24 subunits, each of which folds into a four-alpha-helix bundle. Significant conformational changes were observed at the BC loop and the entrance of the 4-fold symmetry channel in the intermediate and high-iron-bound states, whereas no change was found in the apo and low-iron-bound states. The imidazole rings of His149 at the channel entrance undergo conformational changes that bear resemblance to heme configuration and are directly coupled to axial translocation of Fe ions through the 4-fold channel. Our results provide the first structural evidence of the translocation of Fe ions through the 4-fold channel in prokaryotes and the transition from a protein-dominated process to a mineral-surface-dominated process during biomineralization. The crystal structure of ferritin from Helicobacter pylori reveals unusual conformational changes for iron uptake.,Cho KJ, Shin HJ, Lee JH, Kim KJ, Park SS, Lee Y, Lee C, Park SS, Kim KH J Mol Biol. 2009 Jul 3;390(1):83-98. Epub 2009 May 7. PMID:19427319 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ferritin

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