2ja3: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
The ubiquitous CBS domains, which are found as part of cytoplasmic domains, in the ClC family of chloride channels and transporters, have previously, been identified as building blocks for regulatory nucleotide-binding, sites. Here we report the structures of the cytoplasmic domain of the, human transporter ClC-5 in complex with ATP and ADP. The nucleotides bind, to a specific site in the protein. As determined by equilibrium dialysis, the affinities for ATP, ADP and AMP are in the high micromolar range., Point mutations that interfere with nucleotide binding change the, transport behavior of a ClC-5 mutant expressed in Xenopus laevis oocytes., Our results establish the structural and energetic basis for the, interaction of ClC-5 with nucleotides and provide a framework for future, . | The ubiquitous CBS domains, which are found as part of cytoplasmic domains, in the ClC family of chloride channels and transporters, have previously, been identified as building blocks for regulatory nucleotide-binding, sites. Here we report the structures of the cytoplasmic domain of the, human transporter ClC-5 in complex with ATP and ADP. The nucleotides bind, to a specific site in the protein. As determined by equilibrium dialysis, the affinities for ATP, ADP and AMP are in the high micromolar range., Point mutations that interfere with nucleotide binding change the, transport behavior of a ClC-5 mutant expressed in Xenopus laevis oocytes., Our results establish the structural and energetic basis for the, interaction of ClC-5 with nucleotides and provide a framework for future, investigations. | ||
==About this Structure== | ==About this Structure== | ||
2JA3 is a | 2JA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JA3 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 34: | Line 34: | ||
[[Category: voltage-gated channel]] | [[Category: voltage-gated channel]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:29:58 2007'' | ||
