1u5d: Difference between revisions

Revision as of 14:47, 25 December 2014

Crystal Structure of the PH domain of SKAP55

Structural highlights

1u5d is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	SO4
Related:	1u5g, 1u5e, 1u5f
Gene:	HSSKAP55 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[SKAP1_HUMAN] Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Kang H, Freund C, Duke-Cohan JS, Musacchio A, Wagner G, Rudd CE. SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55. EMBO J. 2000 Jun 15;19(12):2889-99. PMID:10856234 doi:https://dx.doi.org/10.1093/emboj/19.12.2889
↑ Wu L, Fu J, Shen SH. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol Cell Biol. 2002 Apr;22(8):2673-86. PMID:11909961
↑ Wang H, Moon EY, Azouz A, Wu X, Smith A, Schneider H, Hogg N, Rudd CE. SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat Immunol. 2003 Apr;4(4):366-74. Epub 2003 Mar 24. PMID:12652296 doi:https://dx.doi.org/10.1038/ni913
↑ Jo EK, Wang H, Rudd CE. An essential role for SKAP-55 in LFA-1 clustering on T cells that cannot be substituted by SKAP-55R. J Exp Med. 2005 Jun 6;201(11):1733-9. PMID:15939789 doi:https://dx.doi.org/10.1084/jem.20042577
↑ Kliche S, Breitling D, Togni M, Pusch R, Heuer K, Wang X, Freund C, Kasirer-Friede A, Menasche G, Koretzky GA, Schraven B. The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1. Mol Cell Biol. 2006 Oct;26(19):7130-44. PMID:16980616 doi:https://dx.doi.org/26/19/7130

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OCA

[1] Kang H, Freund C, Duke-Cohan JS, Musacchio A, Wagner G, Rudd CE. SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55. EMBO J. 2000 Jun 15;19(12):2889-99. PMID:10856234 doi:https://dx.doi.org/10.1093/emboj/19.12.2889

[2] Wu L, Fu J, Shen SH. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol Cell Biol. 2002 Apr;22(8):2673-86. PMID:11909961

[3] Wang H, Moon EY, Azouz A, Wu X, Smith A, Schneider H, Hogg N, Rudd CE. SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat Immunol. 2003 Apr;4(4):366-74. Epub 2003 Mar 24. PMID:12652296 doi:https://dx.doi.org/10.1038/ni913

[4] Jo EK, Wang H, Rudd CE. An essential role for SKAP-55 in LFA-1 clustering on T cells that cannot be substituted by SKAP-55R. J Exp Med. 2005 Jun 6;201(11):1733-9. PMID:15939789 doi:https://dx.doi.org/10.1084/jem.20042577

[5] Kliche S, Breitling D, Togni M, Pusch R, Heuer K, Wang X, Freund C, Kasirer-Friede A, Menasche G, Koretzky GA, Schraven B. The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1. Mol Cell Biol. 2006 Oct;26(19):7130-44. PMID:16980616 doi:https://dx.doi.org/26/19/7130

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[2]

[3]

[4]

[5]

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1u5d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U5D FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u5g|1u5g]], [[1u5e|1u5e]], [[1u5f|1u5f]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u5g|1u5g]], [[1u5e|1u5e]], [[1u5f|1u5f]]</td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HSSKAP55 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HSSKAP55 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u5d RCSB], [http://www.ebi.ac.uk/pdbsum/1u5d PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u5d RCSB], [http://www.ebi.ac.uk/pdbsum/1u5d PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/SKAP1_HUMAN SKAP1_HUMAN]] Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.<ref>PMID:10856234</ref> <ref>PMID:11909961</ref> <ref>PMID:12652296</ref> <ref>PMID:15939789</ref> <ref>PMID:16980616</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 18: / Line 20: @@
 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 <div style="clear:both"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Eck, M J.]]
+[[Category: Eck, M J]]
-[[Category: Neel, B G.]]
+[[Category: Neel, B G]]
-[[Category: Swanson, K D.]]
+[[Category: Swanson, K D]]
-[[Category: Tang, Y.]]
+[[Category: Tang, Y]]
 [[Category: Ph domain]]
 [[Category: Signaling protein]]

1u5d: Difference between revisions

Revision as of 14:47, 25 December 2014

Crystal Structure of the PH domain of SKAP55

Structural highlights

Function

Evolutionary Conservation

References

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